Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11327 | 5' | -59.1 | NC_003084.1 | + | 20200 | 0.66 | 0.671444 |
Target: 5'- aGUCGC-UGUCCCugUCCCaagucgUCGGUGg -3' miRNA: 3'- aCAGCGuGCGGGGugAGGG------GGUUACa -5' |
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11327 | 5' | -59.1 | NC_003084.1 | + | 22038 | 0.66 | 0.661187 |
Target: 5'- cGUacaGCuCGCCCCGCUCgucgUCCCGGUcGUa -3' miRNA: 3'- aCAg--CGuGCGGGGUGAG----GGGGUUA-CA- -5' |
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11327 | 5' | -59.1 | NC_003084.1 | + | 35953 | 0.69 | 0.528582 |
Target: 5'- cGUCGCAguCGCUgcaCCACUCCCaCCAu--- -3' miRNA: 3'- aCAGCGU--GCGG---GGUGAGGG-GGUuaca -5' |
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11327 | 5' | -59.1 | NC_003084.1 | + | 40682 | 1.06 | 0.001457 |
Target: 5'- cUGUCGCACGCCCCACUCCCCCAAUGUc -3' miRNA: 3'- -ACAGCGUGCGGGGUGAGGGGGUUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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