Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11328 | 3' | -53.8 | NC_003084.1 | + | 23507 | 0.66 | 0.919467 |
Target: 5'- --cGCaCGUACaggUGGCACC-GCUGGUg -3' miRNA: 3'- cuuCGcGCAUGg--AUCGUGGaUGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 74775 | 0.66 | 0.919467 |
Target: 5'- uGAGGaacgaGCGgGCCgcccGCACCUuccGCUGGUu -3' miRNA: 3'- -CUUCg----CGCaUGGau--CGUGGA---UGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 67239 | 0.66 | 0.919467 |
Target: 5'- uGAGCGaGUucg-AGCGCCaACUGGCg -3' miRNA: 3'- cUUCGCgCAuggaUCGUGGaUGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 77812 | 0.66 | 0.907129 |
Target: 5'- --uGCGCGUuaccguaggACCaUAGCACC-ACaaugUGGCg -3' miRNA: 3'- cuuCGCGCA---------UGG-AUCGUGGaUG----ACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 46454 | 0.66 | 0.896516 |
Target: 5'- -uAGCGCGUACCaguUGGUgcgcgaauacagcucGCCaACUGGg -3' miRNA: 3'- cuUCGCGCAUGG---AUCG---------------UGGaUGACCg -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 7389 | 0.67 | 0.886698 |
Target: 5'- cGGAGCGaCGa--CUGGUG-CUGCUGGCa -3' miRNA: 3'- -CUUCGC-GCaugGAUCGUgGAUGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 69990 | 0.68 | 0.84529 |
Target: 5'- --uGCGCGUACUUuugcaggAGCGCCgcuucguccacCUGGCc -3' miRNA: 3'- cuuCGCGCAUGGA-------UCGUGGau---------GACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 10484 | 0.68 | 0.821839 |
Target: 5'- uGGAGCGUGUugGCCUGGUccacACCgcgGCUcaGGUg -3' miRNA: 3'- -CUUCGCGCA--UGGAUCG----UGGa--UGA--CCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 25011 | 0.68 | 0.803571 |
Target: 5'- aGggGCGUGcacgACCUGGCgugcucuuGgCUugUGGCc -3' miRNA: 3'- -CuuCGCGCa---UGGAUCG--------UgGAugACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 1261 | 0.68 | 0.803571 |
Target: 5'- uGAAGCcaaGC-UACCUGGaCACCUcggccgACUGGUg -3' miRNA: 3'- -CUUCG---CGcAUGGAUC-GUGGA------UGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 20690 | 0.69 | 0.794176 |
Target: 5'- uGGAGUuuGCGUGCCguuGCGCUugcaUGCUGaGCg -3' miRNA: 3'- -CUUCG--CGCAUGGau-CGUGG----AUGAC-CG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 94294 | 0.69 | 0.766063 |
Target: 5'- uGAGCGCGUAugcauCgUAGCACCggaggguugugugccACUGGUg -3' miRNA: 3'- cUUCGCGCAU-----GgAUCGUGGa--------------UGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 105793 | 0.71 | 0.682543 |
Target: 5'- aGAAGUGCccaagcUACCUGGaCACCUcggccgACUGGUg -3' miRNA: 3'- -CUUCGCGc-----AUGGAUC-GUGGA------UGACCG- -5' |
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11328 | 3' | -53.8 | NC_003084.1 | + | 41030 | 1.12 | 0.001683 |
Target: 5'- uGAAGCGCGUACCUAGCACCUACUGGCg -3' miRNA: 3'- -CUUCGCGCAUGGAUCGUGGAUGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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