Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11328 | 5' | -49.2 | NC_003084.1 | + | 70478 | 0.66 | 0.995153 |
Target: 5'- gCACCUAUAgcaacuCGAUGGuACcgGCGCuGAu -3' miRNA: 3'- gGUGGAUAUauu---GCUACC-UG--CGCG-CU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 60615 | 0.66 | 0.995153 |
Target: 5'- aCCuCCUGUugcGCGAuuUGGACGCccGUGAc -3' miRNA: 3'- -GGuGGAUAuauUGCU--ACCUGCG--CGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 55219 | 0.67 | 0.992392 |
Target: 5'- aCCACCg----GGCGGUGGAC-UGCa- -3' miRNA: 3'- -GGUGGauauaUUGCUACCUGcGCGcu -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 71462 | 0.67 | 0.992392 |
Target: 5'- aCACCgaugGUGACacUGGAUgugGCGCGAg -3' miRNA: 3'- gGUGGaua-UAUUGcuACCUG---CGCGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 103631 | 0.68 | 0.986912 |
Target: 5'- aCCGCCgugagcauUGUAACGAUGaGCaGgGCGAu -3' miRNA: 3'- -GGUGGau------AUAUUGCUACcUG-CgCGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 16065 | 0.68 | 0.983226 |
Target: 5'- uUCACCc--GUAACGGUGGugGUgaagGUGAa -3' miRNA: 3'- -GGUGGauaUAUUGCUACCugCG----CGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 64529 | 0.68 | 0.983226 |
Target: 5'- gCUACCUAacgGUGGaccugcccagcuCGAUGGAauuuUGCGCGAa -3' miRNA: 3'- -GGUGGAUa--UAUU------------GCUACCU----GCGCGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 40186 | 0.69 | 0.976294 |
Target: 5'- cUCACUc---UAGCGAcUGGACGCGCa- -3' miRNA: 3'- -GGUGGauauAUUGCU-ACCUGCGCGcu -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 1424 | 0.69 | 0.976294 |
Target: 5'- cCCACgCUAg--AGUGGUGGACGCGUu- -3' miRNA: 3'- -GGUG-GAUauaUUGCUACCUGCGCGcu -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 18486 | 0.73 | 0.839366 |
Target: 5'- aCCGCCaagcUGUAucUAGCGGUGGACGUaaacguagucgaucGCGAu -3' miRNA: 3'- -GGUGG----AUAU--AUUGCUACCUGCG--------------CGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 90263 | 0.75 | 0.786878 |
Target: 5'- cCCACCagcgGUGUAACGGUGGAC-C-CGAa -3' miRNA: 3'- -GGUGGa---UAUAUUGCUACCUGcGcGCU- -5' |
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11328 | 5' | -49.2 | NC_003084.1 | + | 40995 | 1.13 | 0.006418 |
Target: 5'- aCCACCUAUAUAACGAUGGACGCGCGAu -3' miRNA: 3'- -GGUGGAUAUAUUGCUACCUGCGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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