Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11329 | 3' | -51 | NC_003084.1 | + | 28021 | 0.66 | 0.983524 |
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Target: 5'- uCGaGUUUGACAAUUUGGGCAAggUGUCgCUg -3' miRNA: 3'- -GC-CGAGCUGUUGAACCUGUU--GUAG-GA- -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 6885 | 0.66 | 0.976686 |
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Target: 5'- uCGGCUgGACAcCUUGcGGCA-CAUgCUg -3' miRNA: 3'- -GCCGAgCUGUuGAAC-CUGUuGUAgGA- -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 33971 | 0.66 | 0.976686 |
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Target: 5'- uCGGCgUUGAgcugaAugUUGGGCAGCAgcUCCa -3' miRNA: 3'- -GCCG-AGCUg----UugAACCUGUUGU--AGGa -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 67731 | 0.66 | 0.973999 |
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Target: 5'- uCGGUg-GGCAACUUGGACgAGCucaCCa -3' miRNA: 3'- -GCCGagCUGUUGAACCUG-UUGua-GGa -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 48640 | 0.69 | 0.910143 |
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Target: 5'- uGGUUCGGugguGCUUGGGCAAaaauuucCAUCCUc -3' miRNA: 3'- gCCGAGCUgu--UGAACCUGUU-------GUAGGA- -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 7467 | 0.7 | 0.897555 |
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Target: 5'- gGGCUCGugcuCGAgUUGGGCAuCAUCa- -3' miRNA: 3'- gCCGAGCu---GUUgAACCUGUuGUAGga -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 67141 | 0.7 | 0.897555 |
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Target: 5'- aCGGCUUGuACGAC-UGGGCAGagcuuUCCUc -3' miRNA: 3'- -GCCGAGC-UGUUGaACCUGUUgu---AGGA- -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 99978 | 0.72 | 0.798146 |
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Target: 5'- uGGCUgGACGGCUcGGcCAACAcgUCCg -3' miRNA: 3'- gCCGAgCUGUUGAaCCuGUUGU--AGGa -5' |
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| 11329 | 3' | -51 | NC_003084.1 | + | 41825 | 1.09 | 0.005759 |
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Target: 5'- cCGGCUCGACAACUUGGACAACAUCCUg -3' miRNA: 3'- -GCCGAGCUGUUGAACCUGUUGUAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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