Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11331 | 5' | -59.7 | NC_003084.1 | + | 15789 | 0.66 | 0.620006 |
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Target: 5'- cUC-ACGCGCAGCACgUCC---GGCAu -3' miRNA: 3'- cAGaUGCGCGUCGUGgAGGgagCCGU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 10383 | 0.66 | 0.620006 |
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Target: 5'- uUCgaguCGUGCGGUACa-CCUUCGGCGa -3' miRNA: 3'- cAGau--GCGCGUCGUGgaGGGAGCCGU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 88412 | 0.67 | 0.567774 |
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Target: 5'- -aUUGCGCagauagaGguGCACCUCgCUCGGUu -3' miRNA: 3'- caGAUGCG-------CguCGUGGAGgGAGCCGu -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 84988 | 0.68 | 0.538545 |
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Target: 5'- uUCUgcaACGCGaAGCAC--CCCUCGGCGa -3' miRNA: 3'- cAGA---UGCGCgUCGUGgaGGGAGCCGU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 17374 | 0.68 | 0.538545 |
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Target: 5'- cGUCUAuCGUgaagcgaaGCAGCGCCUCaacuuCCUCGGgCGu -3' miRNA: 3'- -CAGAU-GCG--------CGUCGUGGAG-----GGAGCC-GU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 53025 | 0.68 | 0.508869 |
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Target: 5'- uGUUauCGCGCAGCACCa-CCUgGGCu -3' miRNA: 3'- -CAGauGCGCGUCGUGGagGGAgCCGu -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 30400 | 0.7 | 0.398352 |
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Target: 5'- ---gAUGCGguGCACCUCCaaCUCgGGCAc -3' miRNA: 3'- cagaUGCGCguCGUGGAGG--GAG-CCGU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 25984 | 0.72 | 0.297442 |
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Target: 5'- aUCaGgGUGCAGCACCagaCCUCGGCGu -3' miRNA: 3'- cAGaUgCGCGUCGUGGag-GGAGCCGU- -5' |
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| 11331 | 5' | -59.7 | NC_003084.1 | + | 44043 | 1.08 | 0.000905 |
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Target: 5'- aGUCUACGCGCAGCACCUCCCUCGGCAa -3' miRNA: 3'- -CAGAUGCGCGUCGUGGAGGGAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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