Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11332 | 5' | -53 | NC_003084.1 | + | 23117 | 0.66 | 0.932128 |
Target: 5'- aAGgGCGGCgucaagacguacCCGAUGUUGGCGcucAACAa -3' miRNA: 3'- gUCgCGCCGa-----------GGUUACAGUCGU---UUGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 47505 | 0.66 | 0.927738 |
Target: 5'- uGGCGCugcacuuuGGUUCCcGUGUCAGU--GCGg -3' miRNA: 3'- gUCGCG--------CCGAGGuUACAGUCGuuUGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 23613 | 0.67 | 0.89643 |
Target: 5'- uCAGCGuCGaGCUCUccaauuGUGUgGGCAGGCu -3' miRNA: 3'- -GUCGC-GC-CGAGGu-----UACAgUCGUUUGu -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 41192 | 0.67 | 0.89643 |
Target: 5'- aCAGCGCGGUcuaacgcuUCCAGucucUGUUcGCAGugAa -3' miRNA: 3'- -GUCGCGCCG--------AGGUU----ACAGuCGUUugU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 2252 | 0.67 | 0.89643 |
Target: 5'- cUAGcCGCacaGCUCCAAUGUUGuGCGGGCGg -3' miRNA: 3'- -GUC-GCGc--CGAGGUUACAGU-CGUUUGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 61288 | 0.67 | 0.882078 |
Target: 5'- uCAGCuGCGGCagcaCGAUGaUCAGCGuauGCAu -3' miRNA: 3'- -GUCG-CGCCGag--GUUAC-AGUCGUu--UGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 79752 | 0.68 | 0.850437 |
Target: 5'- uUAGCGUccgGGCUgCCGuuguUGUCAGCGGAa- -3' miRNA: 3'- -GUCGCG---CCGA-GGUu---ACAGUCGUUUgu -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 27257 | 0.69 | 0.815254 |
Target: 5'- gCGGUGCGGUacgCCGAUGUUAGaaauuuAACAa -3' miRNA: 3'- -GUCGCGCCGa--GGUUACAGUCgu----UUGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 53517 | 0.7 | 0.781025 |
Target: 5'- gCAGCGCGGCuUCCAAaccuccaccaucgcGUCacgggccugGGCAAACAc -3' miRNA: 3'- -GUCGCGCCG-AGGUUa-------------CAG---------UCGUUUGU- -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 13926 | 0.71 | 0.683786 |
Target: 5'- uUAGCGCGGCaaguguUgCGAUGUCGGCcuGAGCc -3' miRNA: 3'- -GUCGCGCCG------AgGUUACAGUCG--UUUGu -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 42094 | 0.74 | 0.512528 |
Target: 5'- aAGUGCGGCUCCAG-GUCAGgCAu--- -3' miRNA: 3'- gUCGCGCCGAGGUUaCAGUC-GUuugu -5' |
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11332 | 5' | -53 | NC_003084.1 | + | 44148 | 1.09 | 0.003169 |
Target: 5'- cCAGCGCGGCUCCAAUGUCAGCAAACAg -3' miRNA: 3'- -GUCGCGCCGAGGUUACAGUCGUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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