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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 11335 | 3' | -53.2 | NC_003084.1 | + | 37800 | 0.78 | 0.356729 |
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Target: 5'- ---uUUAGCACCGCaAAUGugGGCCCc -3' miRNA: 3'- uuacAAUCGUGGUGgUUGCugCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49673 | 0.78 | 0.324659 |
|
Target: 5'- aAAUGUUGGCACCACCAccccagcucACcgguACGGCUCa -3' miRNA: 3'- -UUACAAUCGUGGUGGU---------UGc---UGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49584 | 0.89 | 0.075714 |
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Target: 5'- gAAUGcUAGCACgACUAACGACGGCCCc -3' miRNA: 3'- -UUACaAUCGUGgUGGUUGCUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49503 | 0.9 | 0.062158 |
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Target: 5'- aAAUGcUAGCACCACCAACuACGGCCCc -3' miRNA: 3'- -UUACaAUCGUGGUGGUUGcUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 68425 | 0.94 | 0.034153 |
|
Target: 5'- -uUGUUGGCACCACCGACuACGGCCCc -3' miRNA: 3'- uuACAAUCGUGGUGGUUGcUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 68682 | 0.97 | 0.020331 |
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Target: 5'- cAGUGUUAGCACCACCGACaACGGCCCc -3' miRNA: 3'- -UUACAAUCGUGGUGGUUGcUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49249 | 0.98 | 0.018641 |
|
Target: 5'- aAAUGUUAGCACCACCAACuACGGCCCc -3' miRNA: 3'- -UUACAAUCGUGGUGGUUGcUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49418 | 0.98 | 0.018641 |
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Target: 5'- aAAUGUUAGCACCACCAACuACGGCCCc -3' miRNA: 3'- -UUACAAUCGUGGUGGUUGcUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 68507 | 1.06 | 0.005344 |
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Target: 5'- -cUGUUAGCACCACCAACGACGGCCCc -3' miRNA: 3'- uuACAAUCGUGGUGGUUGCUGCCGGG- -5' |
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| 11335 | 3' | -53.2 | NC_003084.1 | + | 49334 | 1.09 | 0.003352 |
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Target: 5'- aAAUGUUAGCACCACCAACGACGGCCCc -3' miRNA: 3'- -UUACAAUCGUGGUGGUUGCUGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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