Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11338 | 5' | -53.5 | NC_003084.1 | + | 49628 | 0.68 | 0.804629 |
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Target: 5'- --aAGUucACCACUGgcaUGCGCAGUGg -3' miRNA: 3'- auaUCG--UGGUGGCaugACGCGUCACg -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 46263 | 0.71 | 0.682726 |
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Target: 5'- gUAUAGCACCGgUcgAgaGUGCGGUGCg -3' miRNA: 3'- -AUAUCGUGGUgGcaUgaCGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 42463 | 0.66 | 0.901973 |
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Target: 5'- --cGGCACgACCgGUGCU-CGCGGUuauGCg -3' miRNA: 3'- auaUCGUGgUGG-CAUGAcGCGUCA---CG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 37629 | 0.68 | 0.83278 |
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Target: 5'- aAUAGCACCACCGgACcguugaccguaacaUGguugagcugacgaauUGCAGUGCc -3' miRNA: 3'- aUAUCGUGGUGGCaUG--------------AC---------------GCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 35246 | 0.75 | 0.442529 |
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Target: 5'- gGUAGCACCACUugaGCaUGUGCGGUGUc -3' miRNA: 3'- aUAUCGUGGUGGca-UG-ACGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 33630 | 0.72 | 0.59654 |
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Target: 5'- --aGGCGCUuugUCGUGCaGUGCGGUGCg -3' miRNA: 3'- auaUCGUGGu--GGCAUGaCGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 31861 | 0.66 | 0.920846 |
|
Target: 5'- gAUAGCACCACCGcaauCU-CGaauauGUGCu -3' miRNA: 3'- aUAUCGUGGUGGCau--GAcGCgu---CACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 13688 | 0.74 | 0.501876 |
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Target: 5'- --cGGCACCGCCGuUAC-GUGCAcauGUGCg -3' miRNA: 3'- auaUCGUGGUGGC-AUGaCGCGU---CACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 10857 | 0.69 | 0.785562 |
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Target: 5'- --cAGCGguUCACCGUACUGUGCuuccGCa -3' miRNA: 3'- auaUCGU--GGUGGCAUGACGCGuca-CG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 3299 | 0.66 | 0.920846 |
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Target: 5'- --gAGCuCCGCUgGUGCUGC-UGGUGCu -3' miRNA: 3'- auaUCGuGGUGG-CAUGACGcGUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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