Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11338 | 5' | -53.5 | NC_003084.1 | + | 10857 | 0.69 | 0.785562 |
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Target: 5'- --cAGCGguUCACCGUACUGUGCuuccGCa -3' miRNA: 3'- auaUCGU--GGUGGCAUGACGCGuca-CG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 46263 | 0.71 | 0.682726 |
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Target: 5'- gUAUAGCACCGgUcgAgaGUGCGGUGCg -3' miRNA: 3'- -AUAUCGUGGUgGcaUgaCGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 80738 | 0.71 | 0.667714 |
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Target: 5'- -uUGGUAaucauguucaucaCACCGUAUUGCGUGGUGUa -3' miRNA: 3'- auAUCGUg------------GUGGCAUGACGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 33630 | 0.72 | 0.59654 |
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Target: 5'- --aGGCGCUuugUCGUGCaGUGCGGUGCg -3' miRNA: 3'- auaUCGUGGu--GGCAUGaCGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 67203 | 0.72 | 0.585804 |
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Target: 5'- --gAGUccACCACCGUGauCUGCGCGucgguGUGCg -3' miRNA: 3'- auaUCG--UGGUGGCAU--GACGCGU-----CACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 55004 | 0.72 | 0.585804 |
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Target: 5'- gUGUAGCACC--UGUugUGCGCacaauGGUGCu -3' miRNA: 3'- -AUAUCGUGGugGCAugACGCG-----UCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 13688 | 0.74 | 0.501876 |
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Target: 5'- --cGGCACCGCCGuUAC-GUGCAcauGUGCg -3' miRNA: 3'- auaUCGUGGUGGC-AUGaCGCGU---CACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 35246 | 0.75 | 0.442529 |
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Target: 5'- gGUAGCACCACUugaGCaUGUGCGGUGUc -3' miRNA: 3'- aUAUCGUGGUGGca-UG-ACGCGUCACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 64572 | 0.75 | 0.441573 |
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Target: 5'- --gAGCACCACCGUAgCUagaucggGCGCuuuuGUGCu -3' miRNA: 3'- auaUCGUGGUGGCAU-GA-------CGCGu---CACG- -5' |
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| 11338 | 5' | -53.5 | NC_003084.1 | + | 53102 | 1.11 | 0.00202 |
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Target: 5'- uUAUAGCACCACCGUACUGCGCAGUGCa -3' miRNA: 3'- -AUAUCGUGGUGGCAUGACGCGUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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