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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1134 | 3' | -46.2 | NC_000942.1 | + | 13241 | 0.67 | 0.930218 |
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Target: 5'- aAGCcGAGAgAGGGAGCCGGAggaccaauuauuagAGCGUu -3' miRNA: 3'- -UCGaCUUUaUCUUUUGGUCU--------------UCGCGc -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 23812 | 0.67 | 0.94895 |
|
Target: 5'- uGCUGGAGUAGAcAGACguCGGcGGCGUu -3' miRNA: 3'- uCGACUUUAUCU-UUUG--GUCuUCGCGc -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 15708 | 0.67 | 0.94895 |
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Target: 5'- cAGUUGcuuAGUAGAGGuugugugccccGCCGG-GGCGCGa -3' miRNA: 3'- -UCGACu--UUAUCUUU-----------UGGUCuUCGCGC- -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 22569 | 0.67 | 0.943671 |
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Target: 5'- cAGgUGGGcaGGAAAcACCAGAcGGCGCa -3' miRNA: 3'- -UCgACUUuaUCUUU-UGGUCU-UCGCGc -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 19155 | 0.67 | 0.938047 |
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Target: 5'- cGGCUGA------AAACgGGGAGCGCa -3' miRNA: 3'- -UCGACUuuaucuUUUGgUCUUCGCGc -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 9489 | 0.69 | 0.852613 |
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Target: 5'- aGGaggGGGAUAGGAGuacaacgGCCAGuGGCGCGu -3' miRNA: 3'- -UCga-CUUUAUCUUU-------UGGUCuUCGCGC- -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 560 | 0.69 | 0.862876 |
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Target: 5'- uGGCaGuAGUAGAAuccuGCCAuGGAGUGCGa -3' miRNA: 3'- -UCGaCuUUAUCUUu---UGGU-CUUCGCGC- -5' |
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| 1134 | 3' | -46.2 | NC_000942.1 | + | 26794 | 1.1 | 0.003438 |
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Target: 5'- cAGCUGAAAUAGAAAACCAGAAGCGCGu -3' miRNA: 3'- -UCGACUUUAUCUUUUGGUCUUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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