miRNA: 3'- -GCCAC-UUGGCaGgCGCAACCCGgUGg -5'
miRNA: 3'- -GCCACU---UGGCAGGCGcaaCCCGGugG- -5'
miRNA: 3'- -GCCACUUG-GCAgGCGca--ACCCGgUGG- -5'
miRNA: 3'- -GCCACUUGGCAGGCGCAACCCGGUGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11342 | 3' | -58 | NC_003084.1 | + | 18086 | 0.72 | 0.464063 |
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Target: 5'- uCGGUGcAGCCGgUgGCGUUGGGUgACg -3' miRNA: 3'- -GCCAC-UUGGCaGgCGCAACCCGgUGg -5' |
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| 11342 | 3' | -58 | NC_003084.1 | + | 81951 | 0.72 | 0.436753 |
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Target: 5'- cCGGUGAcggACCGUCCGU---GGGCUugUc -3' miRNA: 3'- -GCCACU---UGGCAGGCGcaaCCCGGugG- -5' |
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| 11342 | 3' | -58 | NC_003084.1 | + | 59795 | 0.73 | 0.401826 |
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Target: 5'- aCGGUGGACaCGUgCGCacacUGGGCgACCc -3' miRNA: 3'- -GCCACUUG-GCAgGCGca--ACCCGgUGG- -5' |
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| 11342 | 3' | -58 | NC_003084.1 | + | 67775 | 1.11 | 0.001071 |
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Target: 5'- gCGGUGAACCGUCCGCGUUGGGCCACCu -3' miRNA: 3'- -GCCACUUGGCAGGCGCAACCCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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