Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11342 | 5' | -55.4 | NC_003084.1 | + | 33210 | 0.66 | 0.874209 |
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Target: 5'- aCGGUGGccCGugUgaUGGGCGAGgaCGCu -3' miRNA: 3'- aGCCACCc-GUugA--ACCUGCUCgaGUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 33939 | 0.66 | 0.842483 |
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Target: 5'- gUGGUGGuG-AACggGGACGuGCUCAg -3' miRNA: 3'- aGCCACC-CgUUGaaCCUGCuCGAGUg -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 106742 | 0.68 | 0.760065 |
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Target: 5'- uUUGGcGGGUGuACUUGGcccucaacuCGGGCUCACu -3' miRNA: 3'- -AGCCaCCCGU-UGAACCu--------GCUCGAGUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 25619 | 0.69 | 0.719879 |
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Target: 5'- aCGGUGGaCAACgUGGugGuGUUCAg -3' miRNA: 3'- aGCCACCcGUUGaACCugCuCGAGUg -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 91923 | 0.7 | 0.646849 |
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Target: 5'- gUCGGUGGGgu-CgaGGACGAGaaCACg -3' miRNA: 3'- -AGCCACCCguuGaaCCUGCUCgaGUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 40238 | 0.7 | 0.625731 |
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Target: 5'- cUCGG-GGGUggaccucaccaAACUcacggcuagagUGGACGAGCUCGa -3' miRNA: 3'- -AGCCaCCCG-----------UUGA-----------ACCUGCUCGAGUg -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 103834 | 0.72 | 0.501609 |
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Target: 5'- gUGGUauuuGGGCAGCccgGGugGAGCUcCACc -3' miRNA: 3'- aGCCA----CCCGUUGaa-CCugCUCGA-GUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 101035 | 0.73 | 0.472129 |
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Target: 5'- cCGGUGGGgcuCUUGGGgGcGCUCGCa -3' miRNA: 3'- aGCCACCCguuGAACCUgCuCGAGUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 23847 | 0.77 | 0.302192 |
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Target: 5'- cUCGGUGGGCGcUUUGGAUccGGCUUACa -3' miRNA: 3'- -AGCCACCCGUuGAACCUGc-UCGAGUG- -5' |
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| 11342 | 5' | -55.4 | NC_003084.1 | + | 67732 | 1.09 | 0.002048 |
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Target: 5'- uUCGGUGGGCAACUUGGACGAGCUCACc -3' miRNA: 3'- -AGCCACCCGUUGAACCUGCUCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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