Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11343 | 5' | -47.8 | NC_003084.1 | + | 92932 | 0.66 | 0.99802 |
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Target: 5'- uGACCAU-GGUCgUAuucggguuCCAGUUgUGCGg -3' miRNA: 3'- -CUGGUAgCCAGgAUu-------GGUCAAaAUGC- -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 5351 | 0.66 | 0.99762 |
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Target: 5'- uGAUUAcCGGUCCaccuACCGGUggUGCGc -3' miRNA: 3'- -CUGGUaGCCAGGau--UGGUCAaaAUGC- -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 86980 | 0.67 | 0.995281 |
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Target: 5'- cACCAUCGGUUggGccGCCAGUgaUugGa -3' miRNA: 3'- cUGGUAGCCAGgaU--UGGUCAaaAugC- -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 30835 | 0.68 | 0.990034 |
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Target: 5'- uGACCGUCGGUUC--GCCG---UUACGa -3' miRNA: 3'- -CUGGUAGCCAGGauUGGUcaaAAUGC- -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 12490 | 0.72 | 0.925958 |
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Target: 5'- cGCCAUcuggCGGUCCUGACCGcGUUugUUGCc -3' miRNA: 3'- cUGGUA----GCCAGGAUUGGU-CAA--AAUGc -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 50409 | 0.73 | 0.893521 |
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Target: 5'- aACCAagCGGUCCcaGGCCAGUUUgACGa -3' miRNA: 3'- cUGGUa-GCCAGGa-UUGGUCAAAaUGC- -5' |
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| 11343 | 5' | -47.8 | NC_003084.1 | + | 70110 | 1.09 | 0.01264 |
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Target: 5'- gGACCAUCGGUCCUAACCAGUUUUACGu -3' miRNA: 3'- -CUGGUAGCCAGGAUUGGUCAAAAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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