Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11344 | 3' | -54.3 | NC_003084.1 | + | 81548 | 0.67 | 0.84262 |
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Target: 5'- uGGUGCcacAGAUCGACCcugagggacguauaUCcgaagcugcgGACGGCGUCg -3' miRNA: 3'- gCCACG---UCUGGUUGG--------------AG----------UUGCCGUAG- -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 88219 | 0.67 | 0.836679 |
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Target: 5'- uGGUG-AGACCAACCguguCGGC-UCu -3' miRNA: 3'- gCCACgUCUGGUUGGaguuGCCGuAG- -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 91206 | 0.67 | 0.845134 |
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Target: 5'- aCGGUGCAGcuauCCAACC---ACGGCc-- -3' miRNA: 3'- -GCCACGUCu---GGUUGGaguUGCCGuag -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 97510 | 0.7 | 0.711848 |
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Target: 5'- -cGUGCAGGCCGGgCUCAACaGUuUCa -3' miRNA: 3'- gcCACGUCUGGUUgGAGUUGcCGuAG- -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 102669 | 0.69 | 0.742575 |
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Target: 5'- uCGGUGCAGcCCAACguuaagCUCAugACGaGCGUUg -3' miRNA: 3'- -GCCACGUCuGGUUG------GAGU--UGC-CGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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