Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11344 | 3' | -54.3 | NC_003084.1 | + | 39809 | 0.71 | 0.648689 |
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Target: 5'- aGGUGCaccgugAGACCAACCgCAGCGGa--- -3' miRNA: 3'- gCCACG------UCUGGUUGGaGUUGCCguag -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 52599 | 0.72 | 0.595566 |
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Target: 5'- uCGGUGguGACUcaaggugugAGCUUCAagugaGCGGUAUCg -3' miRNA: 3'- -GCCACguCUGG---------UUGGAGU-----UGCCGUAG- -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 77672 | 0.74 | 0.472632 |
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Target: 5'- gCGGUcGUGGAUgGGCUUCAGCGGCAg- -3' miRNA: 3'- -GCCA-CGUCUGgUUGGAGUUGCCGUag -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 74274 | 0.84 | 0.12564 |
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Target: 5'- gCGGguacgucgGCAGACUAACCUCAauguuucccaGCGGCAUCa -3' miRNA: 3'- -GCCa-------CGUCUGGUUGGAGU----------UGCCGUAG- -5' |
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| 11344 | 3' | -54.3 | NC_003084.1 | + | 73306 | 1.09 | 0.00248 |
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Target: 5'- uCGGUGCAGACCAACCUCAACGGCAUCu -3' miRNA: 3'- -GCCACGUCUGGUUGGAGUUGCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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