Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11345 | 3' | -54 | NC_003084.1 | + | 23256 | 0.66 | 0.906241 |
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Target: 5'- gGCGuGCGUGUacGAccgggccuucaagcGGUGCGCGAu- -3' miRNA: 3'- gCGCuUGCACGaaCU--------------CCACGCGCUuu -5' |
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| 11345 | 3' | -54 | NC_003084.1 | + | 91554 | 0.68 | 0.832174 |
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Target: 5'- -cCGGugGUccGCUccugcGAGGUGCGCGAAc -3' miRNA: 3'- gcGCUugCA--CGAa----CUCCACGCGCUUu -5' |
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| 11345 | 3' | -54 | NC_003084.1 | + | 54690 | 0.68 | 0.814352 |
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Target: 5'- gCGgGAACGUGUUc-GGGUGCGuCGAu- -3' miRNA: 3'- -GCgCUUGCACGAacUCCACGC-GCUuu -5' |
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| 11345 | 3' | -54 | NC_003084.1 | + | 95090 | 0.72 | 0.620398 |
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Target: 5'- uCGUGGACGUGCUUGGuGUGCaCGGc- -3' miRNA: 3'- -GCGCUUGCACGAACUcCACGcGCUuu -5' |
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| 11345 | 3' | -54 | NC_003084.1 | + | 20392 | 0.73 | 0.525464 |
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Target: 5'- gGUGAAUGUGg-UGAGGUGCGCa--- -3' miRNA: 3'- gCGCUUGCACgaACUCCACGCGcuuu -5' |
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| 11345 | 3' | -54 | NC_003084.1 | + | 73601 | 1.07 | 0.003835 |
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Target: 5'- aCGCGAACGUGCUUGAGGUGCGCGAAAu -3' miRNA: 3'- -GCGCUUGCACGAACUCCACGCGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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