Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11345 | 5' | -47.5 | NC_003084.1 | + | 73888 | 0.66 | 0.997178 |
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Target: 5'- aUUUGCGCGCAcGUUCAuGAccagggcguucuugcACGAGUu -3' miRNA: 3'- gAAAUGUGCGU-CGAGUuCU---------------UGCUCAu -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 9238 | 0.67 | 0.996632 |
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Target: 5'- uUUUAUACGacuaaaugaaucgaAGCUCGAGAGcCGAGg- -3' miRNA: 3'- gAAAUGUGCg-------------UCGAGUUCUU-GCUCau -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 80368 | 0.67 | 0.99639 |
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Target: 5'- ---cACGCuGCAGCUgCAGGAACuguGGGUGu -3' miRNA: 3'- gaaaUGUG-CGUCGA-GUUCUUG---CUCAU- -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 10464 | 0.67 | 0.995723 |
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Target: 5'- ---cACAcCGCGGCUCAGGuguUGAGg- -3' miRNA: 3'- gaaaUGU-GCGUCGAGUUCuu-GCUCau -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 56089 | 0.68 | 0.991981 |
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Target: 5'- ----gUAUGCGgcGCUCAAGGACGAGc- -3' miRNA: 3'- gaaauGUGCGU--CGAGUUCUUGCUCau -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 84726 | 0.68 | 0.989319 |
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Target: 5'- ---cGCgACGCAcGC-CAAGAugGAGUGg -3' miRNA: 3'- gaaaUG-UGCGU-CGaGUUCUugCUCAU- -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 82342 | 0.69 | 0.977122 |
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Target: 5'- ----uUACGCAGCUUGAGAGgacgcCGGGUAg -3' miRNA: 3'- gaaauGUGCGUCGAGUUCUU-----GCUCAU- -5' |
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| 11345 | 5' | -47.5 | NC_003084.1 | + | 73567 | 1.07 | 0.014707 |
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Target: 5'- gCUUUACACGCAGCUCAAGAACGAGUAc -3' miRNA: 3'- -GAAAUGUGCGUCGAGUUCUUGCUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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