Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11348 | 3' | -50.8 | NC_003084.1 | + | 55933 | 0.71 | 0.817548 |
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Target: 5'- gGAGCGACA---GGCGCUGCuCAa-- -3' miRNA: 3'- aCUCGUUGUaagCCGCGACGuGUaac -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 23049 | 0.72 | 0.788826 |
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Target: 5'- -uAGCGACAUgcguauggUGGUGCUGUACAUUu -3' miRNA: 3'- acUCGUUGUAa-------GCCGCGACGUGUAAc -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 7390 | 0.72 | 0.788826 |
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Target: 5'- gGAGCGACGacUGGUGCUGCugGc-- -3' miRNA: 3'- aCUCGUUGUaaGCCGCGACGugUaac -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 33145 | 0.72 | 0.788826 |
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Target: 5'- gUGAGUAggccGCA-UCGGCGUUGCugGUg- -3' miRNA: 3'- -ACUCGU----UGUaAGCCGCGACGugUAac -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 44675 | 0.73 | 0.727273 |
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Target: 5'- aGAGCAACAUgUGGCGCaacucaaGCACAa-- -3' miRNA: 3'- aCUCGUUGUAaGCCGCGa------CGUGUaac -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 39890 | 0.73 | 0.716614 |
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Target: 5'- uUGAGCGACA--CGGaGCaGCGCAUUGa -3' miRNA: 3'- -ACUCGUUGUaaGCCgCGaCGUGUAAC- -5' |
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| 11348 | 3' | -50.8 | NC_003084.1 | + | 81835 | 1.08 | 0.005902 |
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Target: 5'- cUGAGCAACAUUCGGCGCUGCACAUUGc -3' miRNA: 3'- -ACUCGUUGUAAGCCGCGACGUGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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