Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11349 | 5' | -55.3 | NC_003084.1 | + | 95552 | 0.66 | 0.905932 |
Target: 5'- gGGcCAGGUUGGaCAGGGcuacgGCgaaUACGGu -3' miRNA: 3'- -CCaGUCCAACUaGUCCCa----CGg--GUGCC- -5' |
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11349 | 5' | -55.3 | NC_003084.1 | + | 89393 | 0.67 | 0.82654 |
Target: 5'- aGGcCAGGUcguccagcuugaagcUGGUuucgcCGGGGUGCCacuuGCGGg -3' miRNA: 3'- -CCaGUCCA---------------ACUA-----GUCCCACGGg---UGCC- -5' |
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11349 | 5' | -55.3 | NC_003084.1 | + | 98852 | 0.68 | 0.805421 |
Target: 5'- -uUCAGGUccaGGUCGGGGUGgUC-CGGu -3' miRNA: 3'- ccAGUCCAa--CUAGUCCCACgGGuGCC- -5' |
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11349 | 5' | -55.3 | NC_003084.1 | + | 81903 | 1.12 | 0.001608 |
Target: 5'- cGGUCAGGUUGAUCAGGGUGCCCACGGc -3' miRNA: 3'- -CCAGUCCAACUAGUCCCACGGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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