Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1135 | 3' | -57.5 | NC_000942.1 | + | 30717 | 0.69 | 0.272889 |
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Target: 5'- cGCCAAaggguUGUUUugaaCCGGGCAgGCaGCCAAa -3' miRNA: 3'- -CGGUU-----ACAAG----GGCCCGUgUGgCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29778 | 0.73 | 0.128542 |
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Target: 5'- uGCCaAAUGUUUCCGGG-ACgACUGCCAAu -3' miRNA: 3'- -CGG-UUACAAGGGCCCgUG-UGGCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29919 | 0.78 | 0.052672 |
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Target: 5'- uCCcAUGUcCCCGGGCACACCGCa-- -3' miRNA: 3'- cGGuUACAaGGGCCCGUGUGGCGguu -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29892 | 0.88 | 0.010036 |
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Target: 5'- uCCcAUGUcCCCGGGCACACCGCCAAg -3' miRNA: 3'- cGGuUACAaGGGCCCGUGUGGCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29999 | 0.88 | 0.010036 |
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Target: 5'- uCCcAUGUcCCCGGGCACACCGCCAAg -3' miRNA: 3'- cGGuUACAaGGGCCCGUGUGGCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29965 | 0.91 | 0.0054 |
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Target: 5'- cGCCAagucccAUGUcCCCGGGCACACCGCCAAg -3' miRNA: 3'- -CGGU------UACAaGGGCCCGUGUGGCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29821 | 1.08 | 0.000291 |
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Target: 5'- uGCCAAUGUUCCCGGGCACACCGCCAAg -3' miRNA: 3'- -CGGUUACAAGGGCCCGUGUGGCGGUU- -5' |
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| 1135 | 3' | -57.5 | NC_000942.1 | + | 29864 | 1.08 | 0.000291 |
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Target: 5'- uGCCAAUGUUCCCGGGCACACCGCCAAg -3' miRNA: 3'- -CGGUUACAAGGGCCCGUGUGGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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