Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1135 | 5' | -56.4 | NC_000942.1 | + | 30004 | 0.67 | 0.437573 |
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Target: 5'- gGACuUGgCGGUGUgCCCGGGgACAUgGg -3' miRNA: 3'- -CUG-ACgGUUACAaGGGCCCgUGUGgC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 30031 | 0.67 | 0.437573 |
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Target: 5'- gGACuUGgCGGUGUgCCCGGGgACAUgGg -3' miRNA: 3'- -CUG-ACgGUUACAaGGGCCCgUGUGgC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29950 | 0.73 | 0.161772 |
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Target: 5'- gGACUGgCGGUGUgCCCGGGgACAUgGg -3' miRNA: 3'- -CUGACgGUUACAaGGGCCCgUGUGgC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29796 | 0.78 | 0.071193 |
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Target: 5'- cGACUGCCAAUGUUCCCGGGa------ -3' miRNA: 3'- -CUGACGGUUACAAGGGCCCguguggc -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29992 | 0.78 | 0.077879 |
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Target: 5'- ---nGCCAagucccAUGUcCCCGGGCACACCGc -3' miRNA: 3'- cugaCGGU------UACAaGGGCCCGUGUGGC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29909 | 0.79 | 0.065061 |
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Target: 5'- cACcGCCAagucccAUGUcCCCGGGCACACCGc -3' miRNA: 3'- cUGaCGGU------UACAaGGGCCCGUGUGGC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29962 | 0.79 | 0.065061 |
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Target: 5'- cACcGCCAagucccAUGUcCCCGGGCACACCGc -3' miRNA: 3'- cUGaCGGU------UACAaGGGCCCGUGUGGC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29817 | 1.08 | 0.000397 |
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Target: 5'- cGACUGCCAAUGUUCCCGGGCACACCGc -3' miRNA: 3'- -CUGACGGUUACAAGGGCCCGUGUGGC- -5' |
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| 1135 | 5' | -56.4 | NC_000942.1 | + | 29860 | 1.08 | 0.000397 |
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Target: 5'- cGACUGCCAAUGUUCCCGGGCACACCGc -3' miRNA: 3'- -CUGACGGUUACAAGGGCCCGUGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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