Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11351 | 5' | -51.6 | NC_003084.1 | + | 1037 | 0.66 | 0.966991 |
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Target: 5'- cAGACccacugcUUGUAGCGCUUGGcGuuGGGUa -3' miRNA: 3'- aUCUGuu-----AACAUCGCGAGCCaC--UCCA- -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 81919 | 0.66 | 0.966991 |
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Target: 5'- aGGuCGAUUGUGGC-UUCGGUcAGGUu -3' miRNA: 3'- aUCuGUUAACAUCGcGAGCCAcUCCA- -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 79624 | 0.66 | 0.959796 |
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Target: 5'- gUGGAUGGagcUGUAGCGCUCGGUaccGGc -3' miRNA: 3'- -AUCUGUUa--ACAUCGCGAGCCAcu-CCa -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 91560 | 0.67 | 0.937292 |
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Target: 5'- --cGCAGUUGUGGUGUUgGGUuGGGUu -3' miRNA: 3'- aucUGUUAACAUCGCGAgCCAcUCCA- -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 18077 | 0.7 | 0.820895 |
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Target: 5'- cUGGACAAUUcggugcagccgGUGGCGUUgGGUGAcGGg -3' miRNA: 3'- -AUCUGUUAA-----------CAUCGCGAgCCACU-CCa -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 79847 | 0.71 | 0.752474 |
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Target: 5'- gUGGACGAaggUGUAGUGCaCGGUGcGGUu -3' miRNA: 3'- -AUCUGUUa--ACAUCGCGaGCCACuCCA- -5' |
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| 11351 | 5' | -51.6 | NC_003084.1 | + | 92010 | 1.07 | 0.00617 |
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Target: 5'- uUAGACAAUUGUAGCGCUCGGUGAGGUa -3' miRNA: 3'- -AUCUGUUAACAUCGCGAGCCACUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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