Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11352 | 5' | -53.5 | NC_003084.1 | + | 54138 | 0.66 | 0.924564 |
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Target: 5'- cCGGUGCUGCuGcUGCugucUGCGUUCAc -3' miRNA: 3'- -GCCACGAUG-CuACGuau-GCGCGAGUa -5' |
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| 11352 | 5' | -53.5 | NC_003084.1 | + | 17080 | 0.66 | 0.918725 |
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Target: 5'- cCGGUGCcgcuCGcgGCGUACgGCGCa--- -3' miRNA: 3'- -GCCACGau--GCuaCGUAUG-CGCGagua -5' |
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| 11352 | 5' | -53.5 | NC_003084.1 | + | 43083 | 0.66 | 0.918725 |
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Target: 5'- ---cGCUGCGA---GUugGCGCUCAUg -3' miRNA: 3'- gccaCGAUGCUacgUAugCGCGAGUA- -5' |
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| 11352 | 5' | -53.5 | NC_003084.1 | + | 104655 | 0.68 | 0.837796 |
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Target: 5'- uGG-GCUACGGcUGCGUGagauUGCGCUCc- -3' miRNA: 3'- gCCaCGAUGCU-ACGUAU----GCGCGAGua -5' |
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| 11352 | 5' | -53.5 | NC_003084.1 | + | 27684 | 0.68 | 0.810994 |
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Target: 5'- gGGUGCcgAUGAUGUA---GCGCUCAg -3' miRNA: 3'- gCCACGa-UGCUACGUaugCGCGAGUa -5' |
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| 11352 | 5' | -53.5 | NC_003084.1 | + | 94426 | 1.06 | 0.004904 |
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Target: 5'- cCGGUGCUACGAUGCAUACGCGCUCAUc -3' miRNA: 3'- -GCCACGAUGCUACGUAUGCGCGAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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