Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11358 | 5' | -52.2 | NC_003084.1 | + | 96650 | 0.66 | 0.948087 |
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Target: 5'- -uGGCUGCugagguGGUGGUUGGGCgagCGUUc -3' miRNA: 3'- auUCGACGuu----CUACCAGCCUG---GCAAa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 70232 | 0.66 | 0.927553 |
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Target: 5'- gGAGCUGagguuGGUGGUgaggCGGGCCGa-- -3' miRNA: 3'- aUUCGACguu--CUACCA----GCCUGGCaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 80375 | 0.67 | 0.922326 |
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Target: 5'- -cAGCUGCAGGAacugUGGguguuagaaacgacgCGGGCCGc-- -3' miRNA: 3'- auUCGACGUUCU----ACCa--------------GCCUGGCaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 75794 | 0.67 | 0.91501 |
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Target: 5'- cGAGCUGUacGAGcgGGUCgccaaccGGGCCGg-- -3' miRNA: 3'- aUUCGACG--UUCuaCCAG-------CCUGGCaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 30394 | 0.67 | 0.902621 |
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Target: 5'- gGAGCUGCAGuGUGaGUCGaGGCCa--- -3' miRNA: 3'- aUUCGACGUUcUAC-CAGC-CUGGcaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 47596 | 0.67 | 0.895709 |
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Target: 5'- gGAGCUGaaCGAGcUGGUCGGAaccCCGg-- -3' miRNA: 3'- aUUCGAC--GUUCuACCAGCCU---GGCaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 16258 | 0.68 | 0.857272 |
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Target: 5'- cAAGCUGCAGGAUGccgaGGugCGg-- -3' miRNA: 3'- aUUCGACGUUCUACcag-CCugGCaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 5922 | 0.68 | 0.857272 |
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Target: 5'- gGAGCgGCAAGAUaccguagcgccGGUUGGACCu--- -3' miRNA: 3'- aUUCGaCGUUCUA-----------CCAGCCUGGcaaa -5' |
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| 11358 | 5' | -52.2 | NC_003084.1 | + | 2145 | 1.04 | 0.006837 |
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Target: 5'- uUAAGCUGCAAGAUGGUCGGACCGUUUg -3' miRNA: 3'- -AUUCGACGUUCUACCAGCCUGGCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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