Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11362 | 5' | -51.1 | NC_003084.1 | + | 25921 | 0.66 | 0.966474 |
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Target: 5'- cGGUGa-AGCGCCGGCuGUGUUcgUGu -3' miRNA: 3'- aCCACgcUCGCGGUUG-UACAGuaAUg -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 75253 | 0.66 | 0.96296 |
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Target: 5'- cGGaGCGuGCGCgCGGCAcgGUCAUaGCc -3' miRNA: 3'- aCCaCGCuCGCG-GUUGUa-CAGUAaUG- -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 88476 | 0.67 | 0.959198 |
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Target: 5'- cGGUGUGAggaacuaucuguGCGauuCCAACAUGUCGg--- -3' miRNA: 3'- aCCACGCU------------CGC---GGUUGUACAGUaaug -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 58034 | 0.67 | 0.950902 |
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Target: 5'- cUGGUGC-AGCGCCAAauccUGUUgaUACu -3' miRNA: 3'- -ACCACGcUCGCGGUUgu--ACAGuaAUG- -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 765 | 0.68 | 0.931115 |
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Target: 5'- aGGUcGCGcuGCacaucuucgGCCAACAUGcCGUUACg -3' miRNA: 3'- aCCA-CGCu-CG---------CGGUUGUACaGUAAUG- -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 14578 | 0.68 | 0.919584 |
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Target: 5'- --uUGCGAGgGCCGAUGUGUCcgagagGCa -3' miRNA: 3'- accACGCUCgCGGUUGUACAGuaa---UG- -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 27251 | 0.69 | 0.900243 |
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Target: 5'- aUGGUGCGAGCGCaaguCGgcUCAUcgGCu -3' miRNA: 3'- -ACCACGCUCGCGguu-GUacAGUAa-UG- -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 5978 | 0.69 | 0.900243 |
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Target: 5'- gUGGUGCGAGUGUgcagaauuCAGCGUGauaCGUUGg -3' miRNA: 3'- -ACCACGCUCGCG--------GUUGUACa--GUAAUg -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 70419 | 0.69 | 0.886013 |
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Target: 5'- cUGGUGCGAGUcccguucgaGCuCAACAgugGUCGUg-- -3' miRNA: 3'- -ACCACGCUCG---------CG-GUUGUa--CAGUAaug -5' |
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| 11362 | 5' | -51.1 | NC_003084.1 | + | 12291 | 1.11 | 0.00358 |
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Target: 5'- gUGGUGCGAGCGCCAACAUGUCAUUACg -3' miRNA: 3'- -ACCACGCUCGCGGUUGUACAGUAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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