Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11368 | 3' | -56.9 | NC_003084.1 | + | 68589 | 0.89 | 0.033478 |
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Target: 5'- ----cGGCCCCACGAAAGUGGACCCGg -3' miRNA: 3'- uacacCCGGGGUGUUUUCACCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 68703 | 0.78 | 0.190949 |
|
Target: 5'- ----cGGCCCCACAAAAGUuGACCCGa -3' miRNA: 3'- uacacCCGGGGUGUUUUCAcCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 70286 | 0.66 | 0.76225 |
|
Target: 5'- -gGUGGGCCCCgACAu-GGUG-ACCa- -3' miRNA: 3'- uaCACCCGGGG-UGUuuUCACcUGGgc -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 75596 | 0.67 | 0.709081 |
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Target: 5'- aAUGUuGGCCcaagaauugcgcccCCACucuAGUGGACCCa -3' miRNA: 3'- -UACAcCCGG--------------GGUGuuuUCACCUGGGc -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 80841 | 0.72 | 0.410557 |
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Target: 5'- -gGUGGGCCUCACc-----GGACCCGa -3' miRNA: 3'- uaCACCCGGGGUGuuuucaCCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 91551 | 0.66 | 0.771945 |
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Target: 5'- -aGUuGGCCUCGCAGuuGUGGugUUGg -3' miRNA: 3'- uaCAcCCGGGGUGUUuuCACCugGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 94142 | 0.68 | 0.681159 |
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Target: 5'- cGUGUuGGCCCUuuGguGAGGUGGACCg- -3' miRNA: 3'- -UACAcCCGGGG--UguUUUCACCUGGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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