Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11368 | 3' | -56.9 | NC_003084.1 | + | 37934 | 0.66 | 0.800198 |
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Target: 5'- uUGUGGGgCCCACAuuuGcGGugCUa -3' miRNA: 3'- uACACCCgGGGUGUuuuCaCCugGGc -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 37877 | 0.9 | 0.029918 |
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Target: 5'- uUG-GcGGCCCCACAAAAGUGGACCCGu -3' miRNA: 3'- uACaC-CCGGGGUGUUUUCACCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 37812 | 1.06 | 0.002191 |
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Target: 5'- aAUGUGGGCCCCACAAAAGUGGACCCGu -3' miRNA: 3'- -UACACCCGGGGUGUUUUCACCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 28225 | 0.68 | 0.681159 |
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Target: 5'- ---gGGGCUgCAUAugGGUGGACaCCGa -3' miRNA: 3'- uacaCCCGGgGUGUuuUCACCUG-GGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 24111 | 0.66 | 0.781507 |
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Target: 5'- --aUGGGUUCC-C-AAAGUGGugCCGa -3' miRNA: 3'- uacACCCGGGGuGuUUUCACCugGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 17068 | 0.67 | 0.732479 |
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Target: 5'- ---cGGGCUCauCGGAGGUGGACUCGu -3' miRNA: 3'- uacaCCCGGGguGUUUUCACCUGGGC- -5' |
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| 11368 | 3' | -56.9 | NC_003084.1 | + | 5853 | 0.67 | 0.742508 |
|
Target: 5'- ---cGGGCCCCGCAucAGGUGcuccucGGCCaCGg -3' miRNA: 3'- uacaCCCGGGGUGUu-UUCAC------CUGG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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