Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11370 | 3' | -50.9 | NC_003084.1 | + | 62119 | 0.66 | 0.98432 |
Target: 5'- uGAAAGUuuGGCCCGCGAuaggaGUaaguUUGCGuAGg -3' miRNA: 3'- -CUUUCG--CCGGGCGCU-----CAau--AAUGC-UC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 84937 | 0.66 | 0.98432 |
Target: 5'- uAAAGCGGCU--CGAGUUGgaacuggACGAGu -3' miRNA: 3'- cUUUCGCCGGgcGCUCAAUaa-----UGCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 91916 | 0.66 | 0.98432 |
Target: 5'- cGAGGUGGUCgGUGGGgucgAggACGAGa -3' miRNA: 3'- cUUUCGCCGGgCGCUCaa--UaaUGCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 26462 | 0.66 | 0.977697 |
Target: 5'- -cGAGgGGCUCGCGAGUgug-GCa-- -3' miRNA: 3'- cuUUCgCCGGGCGCUCAauaaUGcuc -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 96607 | 0.67 | 0.969211 |
Target: 5'- ---uGCGGCUgCGCGAccgGUUGggACGGGg -3' miRNA: 3'- cuuuCGCCGG-GCGCU---CAAUaaUGCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 106752 | 0.67 | 0.965588 |
Target: 5'- uGGAGCGGUugggcgaacguauUCGUGAG-UGUUugGAGg -3' miRNA: 3'- cUUUCGCCG-------------GGCGCUCaAUAAugCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 32613 | 0.7 | 0.893001 |
Target: 5'- ---uGCGGUCCGCcGGGUUua-GCGAGc -3' miRNA: 3'- cuuuCGCCGGGCG-CUCAAuaaUGCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 33208 | 0.7 | 0.893001 |
Target: 5'- cGAcGGUGGCCCGUGuGaugg-GCGAGg -3' miRNA: 3'- -CUuUCGCCGGGCGCuCaauaaUGCUC- -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 85688 | 0.72 | 0.791544 |
Target: 5'- cGAGAG-GGCCCGCGAGguggUGCa-- -3' miRNA: 3'- -CUUUCgCCGGGCGCUCaauaAUGcuc -5' |
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11370 | 3' | -50.9 | NC_003084.1 | + | 47684 | 1.09 | 0.006432 |
Target: 5'- aGAAAGCGGCCCGCGAGUUAUUACGAGc -3' miRNA: 3'- -CUUUCGCCGGGCGCUCAAUAAUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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