Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11375 | 5' | -57.1 | NC_003084.1 | + | 72567 | 0.66 | 0.791255 |
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Target: 5'- -gUCGGACGAGuugguuGGUUGGGCG-GaGCGu -3' miRNA: 3'- ugAGCCUGCUC------UUAGCCCGCaC-CGUu -5' |
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| 11375 | 5' | -57.1 | NC_003084.1 | + | 47621 | 0.66 | 0.761745 |
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Target: 5'- --cCGGACGAGAuggcaaaAUCGGGCGUaauccagcgcGGUu- -3' miRNA: 3'- ugaGCCUGCUCU-------UAGCCCGCA----------CCGuu -5' |
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| 11375 | 5' | -57.1 | NC_003084.1 | + | 24733 | 0.67 | 0.730076 |
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Target: 5'- -gUCGGGCGGGucGUCuaccaauugccgcuGGGCGUGcGCGAu -3' miRNA: 3'- ugAGCCUGCUCu-UAG--------------CCCGCAC-CGUU- -5' |
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| 11375 | 5' | -57.1 | NC_003084.1 | + | 68793 | 0.68 | 0.650769 |
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Target: 5'- cACUgGGugGGcacGAGUCGGGCuagagGUGGUAu -3' miRNA: 3'- -UGAgCCugCU---CUUAGCCCG-----CACCGUu -5' |
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| 11375 | 5' | -57.1 | NC_003084.1 | + | 7444 | 0.72 | 0.439489 |
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Target: 5'- gGCUCGGGCuuuGGAUCGGGCucgGGCu- -3' miRNA: 3'- -UGAGCCUGcu-CUUAGCCCGca-CCGuu -5' |
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| 11375 | 5' | -57.1 | NC_003084.1 | + | 53935 | 1.06 | 0.002321 |
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Target: 5'- gACUCGGACGAGAAUCGGGCGUGGCAAg -3' miRNA: 3'- -UGAGCCUGCUCUUAGCCCGCACCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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