Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11379 | 3' | -52.9 | NC_003084.1 | + | 103790 | 0.66 | 0.950357 |
Target: 5'- cCCuGUauaucucuaUGGCGGGCCGguuugaacgcUCUCGCAacgUGg -3' miRNA: 3'- -GGuCA---------AUCGCCUGGC----------AGAGCGUga-AC- -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 35645 | 0.66 | 0.941253 |
Target: 5'- uCUAG-UGGUGGAUCGUacacauuucCGCACUUGu -3' miRNA: 3'- -GGUCaAUCGCCUGGCAga-------GCGUGAAC- -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 77010 | 0.67 | 0.93632 |
Target: 5'- -aAGUucUGGCGuACCGUCUCaGCACa-- -3' miRNA: 3'- ggUCA--AUCGCcUGGCAGAG-CGUGaac -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 75659 | 0.69 | 0.840979 |
Target: 5'- cCCGGUUGGCGacCCG-CUCGUACa-- -3' miRNA: 3'- -GGUCAAUCGCcuGGCaGAGCGUGaac -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 81952 | 0.71 | 0.737808 |
Target: 5'- cCCGGUga-CGGACCGUC-CGUggGCUUGu -3' miRNA: 3'- -GGUCAaucGCCUGGCAGaGCG--UGAAC- -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 46603 | 0.73 | 0.63328 |
Target: 5'- cCCAGUUGGCGaGCUGUaUUCGCGCa-- -3' miRNA: 3'- -GGUCAAUCGCcUGGCA-GAGCGUGaac -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 36074 | 0.77 | 0.449418 |
Target: 5'- aCCAGUUGGCGGugCGgggcUUCGUACa-- -3' miRNA: 3'- -GGUCAAUCGCCugGCa---GAGCGUGaac -5' |
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11379 | 3' | -52.9 | NC_003084.1 | + | 61584 | 1.12 | 0.002864 |
Target: 5'- cCCAGUUAGCGGACCGUCUCGCACUUGg -3' miRNA: 3'- -GGUCAAUCGCCUGGCAGAGCGUGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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