Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11380 | 3' | -54.3 | NC_003084.1 | + | 22091 | 0.67 | 0.857414 |
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Target: 5'- cGCUGUuugaGGCCCUgaAGG-CGAaccgGGCCCa -3' miRNA: 3'- uUGACA----UUGGGAacUCCaGCU----CCGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 58072 | 0.68 | 0.823534 |
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Target: 5'- aGAUUGUAAU---UGAGGUCGAGuacggcGCCCa -3' miRNA: 3'- -UUGACAUUGggaACUCCAGCUC------CGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 61821 | 0.68 | 0.814571 |
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Target: 5'- gGAUUGUccACCUUcaUGGGGuUCGAGaGCCCu -3' miRNA: 3'- -UUGACAu-UGGGA--ACUCC-AGCUC-CGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 88935 | 0.68 | 0.796115 |
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Target: 5'- aAACUGUAucACCCUcgauggUGGGGUC--GGCCa -3' miRNA: 3'- -UUGACAU--UGGGA------ACUCCAGcuCCGGg -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 92005 | 0.69 | 0.76725 |
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Target: 5'- cAAUUGUAGCgCUcggUGAGGUacaacaGGGCCCg -3' miRNA: 3'- -UUGACAUUGgGA---ACUCCAgc----UCCGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 95444 | 0.69 | 0.734156 |
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Target: 5'- aAACUGUucACCCaUGGGGUCcgcgagcguacuguuGGCCCg -3' miRNA: 3'- -UUGACAu-UGGGaACUCCAGcu-------------CCGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 66287 | 0.7 | 0.674739 |
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Target: 5'- cGACggagAACCCggUGGGGUCG-GGCCa -3' miRNA: 3'- -UUGaca-UUGGGa-ACUCCAGCuCCGGg -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 6908 | 0.74 | 0.457269 |
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Target: 5'- uGCUGgccguggGAUCCUUGAaGcCGAGGCCCg -3' miRNA: 3'- uUGACa------UUGGGAACUcCaGCUCCGGG- -5' |
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| 11380 | 3' | -54.3 | NC_003084.1 | + | 63016 | 1.1 | 0.002146 |
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Target: 5'- uAACUGUAACCCUUGAGGUCGAGGCCCa -3' miRNA: 3'- -UUGACAUUGGGAACUCCAGCUCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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