miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11383 5' -58.7 NC_003084.1 + 15639 0.66 0.676351
Target:  5'- -gCGgUGGAGCuguacaccaaaauGCCGGaCGuGCUGCGCg -3'
miRNA:   3'- aaGCgAUCUCG-------------CGGCCaGU-CGACGUG- -5'
11383 5' -58.7 NC_003084.1 + 96462 0.66 0.64594
Target:  5'- cUCGCacaucUGCCGGUCGGCcuugUGCACg -3'
miRNA:   3'- aAGCGaucucGCGGCCAGUCG----ACGUG- -5'
11383 5' -58.7 NC_003084.1 + 43818 0.67 0.614378
Target:  5'- -cCGuCUGGAugGUG-CGGUCuAGCUGCACa -3'
miRNA:   3'- aaGC-GAUCU--CGCgGCCAG-UCGACGUG- -5'
11383 5' -58.7 NC_003084.1 + 107173 0.69 0.452795
Target:  5'- -gCGCUGuacGAGCGCaCGGUCgaggauGGCUGuCGCg -3'
miRNA:   3'- aaGCGAU---CUCGCG-GCCAG------UCGAC-GUG- -5'
11383 5' -58.7 NC_003084.1 + 100353 0.77 0.161565
Target:  5'- -gCGUUAGGGCGCUGGagCGGCUGCGu -3'
miRNA:   3'- aaGCGAUCUCGCGGCCa-GUCGACGUg -5'
11383 5' -58.7 NC_003084.1 + 65177 1.07 0.001052
Target:  5'- cUUCGCUAGAGCGCCGGUCAGCUGCACg -3'
miRNA:   3'- -AAGCGAUCUCGCGGCCAGUCGACGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.