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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11388 | 3' | -52.8 | NC_003084.1 | + | 107151 | 0.66 | 0.948844 |
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Target: 5'- aGGAUggcUGUCGCGUGguGCGgguuugccgUGCCCAa -3' miRNA: 3'- -CUUA---GCGGCGCGCuuUGCaa-------ACGGGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 528 | 0.66 | 0.944281 |
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Target: 5'- cAGUCGaaGCGCuGAGCGUUgGCCuCAg -3' miRNA: 3'- cUUAGCggCGCGcUUUGCAAaCGG-GU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 73809 | 0.67 | 0.92903 |
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Target: 5'- cAGUCGuuGCgaaucagcgGCGAAAUGUccaGCCCAa -3' miRNA: 3'- cUUAGCggCG---------CGCUUUGCAaa-CGGGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 78514 | 0.7 | 0.809659 |
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Target: 5'- cGGUCGCgCGCGgGGuucGAUGUgcGCCCAa -3' miRNA: 3'- cUUAGCG-GCGCgCU---UUGCAaaCGGGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 61657 | 0.71 | 0.761602 |
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Target: 5'- aGAAUCGCUaUGCGAGAUGgg-GUCCAc -3' miRNA: 3'- -CUUAGCGGcGCGCUUUGCaaaCGGGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 53349 | 0.71 | 0.720825 |
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Target: 5'- ----aGCUGCGCaGAGUGUUUGCCCAg -3' miRNA: 3'- cuuagCGGCGCGcUUUGCAAACGGGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 98220 | 0.72 | 0.67871 |
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Target: 5'- --cUCGgCGCGCGGGAUGUUggugGCaCCAa -3' miRNA: 3'- cuuAGCgGCGCGCUUUGCAAa---CG-GGU- -5' |
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| 11388 | 3' | -52.8 | NC_003084.1 | + | 69603 | 1.08 | 0.004313 |
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Target: 5'- gGAAUCGCCGCGCGAAACGUUUGCCCAc -3' miRNA: 3'- -CUUAGCGGCGCGCUUUGCAAACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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