Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11388 | 5' | -52.2 | NC_003084.1 | + | 46597 | 0.66 | 0.965118 |
Target: 5'- uGGCGAGCUgUAUUcgcgCACcaACUgGUACGc -3' miRNA: 3'- -CCGCUCGGgGUAAa---GUG--UGAgCAUGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 81403 | 0.67 | 0.945015 |
Target: 5'- cGCGAGCCCCAcggUUACaACUUGc--- -3' miRNA: 3'- cCGCUCGGGGUaa-AGUG-UGAGCaugu -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 52229 | 0.67 | 0.9299 |
Target: 5'- -uUGAGUCCCGcgagguggcauaUUUUGCGCUCGUAUg -3' miRNA: 3'- ccGCUCGGGGU------------AAAGUGUGAGCAUGu -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 84775 | 0.69 | 0.853859 |
Target: 5'- gGGCGcacucucAGCgCCCuUUUUACACUCGUcCAg -3' miRNA: 3'- -CCGC-------UCG-GGGuAAAGUGUGAGCAuGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 38630 | 0.7 | 0.837905 |
Target: 5'- cGGC-AGCCCCggUUCcCGCgggUGUGCAg -3' miRNA: 3'- -CCGcUCGGGGuaAAGuGUGa--GCAUGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 11962 | 0.7 | 0.829207 |
Target: 5'- cGGUGAaauuGCaCCCAUUUCAC-C-CGUACGa -3' miRNA: 3'- -CCGCU----CG-GGGUAAAGUGuGaGCAUGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 26581 | 0.71 | 0.79254 |
Target: 5'- cGCGAGCCCCu-----CGCUCGUguugGCAa -3' miRNA: 3'- cCGCUCGGGGuaaaguGUGAGCA----UGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 71372 | 0.72 | 0.71226 |
Target: 5'- aGCGAGCCCCAguuguccgCAUAUcUGUACAc -3' miRNA: 3'- cCGCUCGGGGUaaa-----GUGUGaGCAUGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 97539 | 0.74 | 0.637885 |
Target: 5'- uGGCGGuGCCCCucaaagUCAgccaaACUCGUGCAg -3' miRNA: 3'- -CCGCU-CGGGGuaa---AGUg----UGAGCAUGU- -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 35558 | 0.75 | 0.5527 |
Target: 5'- aGUaGGaCCCCAcUUCACACUCGUGCu -3' miRNA: 3'- cCGcUC-GGGGUaAAGUGUGAGCAUGu -5' |
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11388 | 5' | -52.2 | NC_003084.1 | + | 69638 | 1.11 | 0.003254 |
Target: 5'- uGGCGAGCCCCAUUUCACACUCGUACAa -3' miRNA: 3'- -CCGCUCGGGGUAAAGUGUGAGCAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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