Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11393 | 3' | -55.1 | NC_003084.1 | + | 64008 | 0.66 | 0.886099 |
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Target: 5'- aGCG-CAuccGUaCCCUuCAGGUGUAC-CCGa -3' miRNA: 3'- -CGCuGU---CA-GGGAuGUCCACAUGcGGC- -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 48038 | 0.66 | 0.87896 |
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Target: 5'- cGCGcACAGaaaaauuaucgCCCgugGGGUGUGCGUCGa -3' miRNA: 3'- -CGC-UGUCa----------GGGaugUCCACAUGCGGC- -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 97613 | 0.66 | 0.87896 |
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Target: 5'- uGCG-CGGUCUccagCUACgaGGGUGaaucgGCGCCGa -3' miRNA: 3'- -CGCuGUCAGG----GAUG--UCCACa----UGCGGC- -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 100447 | 0.67 | 0.856191 |
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Target: 5'- gGCGACAGUUgCUGCaucgAGGauaUGaACGCCu -3' miRNA: 3'- -CGCUGUCAGgGAUG----UCC---ACaUGCGGc -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 44558 | 0.69 | 0.735391 |
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Target: 5'- uGUGGUGGUCCUcauuucugggguugUGCAGcGUGUACGCCu -3' miRNA: 3'- -CGCUGUCAGGG--------------AUGUC-CACAUGCGGc -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 105281 | 0.7 | 0.687597 |
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Target: 5'- aGCGA----UCCUGCAuaucGGUGUACGCCGc -3' miRNA: 3'- -CGCUgucaGGGAUGU----CCACAUGCGGC- -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 38629 | 0.72 | 0.562909 |
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Target: 5'- uCGGCAGcCCCgguucccGCGGGUGUGCaGCCc -3' miRNA: 3'- cGCUGUCaGGGa------UGUCCACAUG-CGGc -5' |
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| 11393 | 3' | -55.1 | NC_003084.1 | + | 80757 | 1.11 | 0.001798 |
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Target: 5'- uGCGACAGUCCCUACAGGUGUACGCCGg -3' miRNA: 3'- -CGCUGUCAGGGAUGUCCACAUGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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