Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11393 | 5' | -54.5 | NC_003084.1 | + | 58298 | 0.66 | 0.876858 |
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Target: 5'- cACCGacucUGgGCUCAauaauaUgCGCGCGGUUGUg -3' miRNA: 3'- -UGGC----ACgCGAGUa-----AgGCGUGCCAAUA- -5' |
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| 11393 | 5' | -54.5 | NC_003084.1 | + | 39627 | 0.66 | 0.861409 |
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Target: 5'- cGCCGUuuGCGCagGUUCCGCACc----- -3' miRNA: 3'- -UGGCA--CGCGagUAAGGCGUGccaaua -5' |
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| 11393 | 5' | -54.5 | NC_003084.1 | + | 23863 | 0.67 | 0.836565 |
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Target: 5'- cACCGUGgGgUCcacguacucguGUUCCaGCAUGGUUGUu -3' miRNA: 3'- -UGGCACgCgAG-----------UAAGG-CGUGCCAAUA- -5' |
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| 11393 | 5' | -54.5 | NC_003084.1 | + | 107045 | 0.68 | 0.807143 |
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Target: 5'- gACCGUGCGCUCGUacagcgcaauauaaUCCuccUugGGUUc- -3' miRNA: 3'- -UGGCACGCGAGUA--------------AGGc--GugCCAAua -5' |
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| 11393 | 5' | -54.5 | NC_003084.1 | + | 36747 | 0.69 | 0.711125 |
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Target: 5'- aGCUGUGCGaaaaAUUCCGgAUGGUUAa -3' miRNA: 3'- -UGGCACGCgag-UAAGGCgUGCCAAUa -5' |
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| 11393 | 5' | -54.5 | NC_003084.1 | + | 80791 | 1.05 | 0.004559 |
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Target: 5'- aACCGUGCGCUCAUUCCGCACGGUUAUc -3' miRNA: 3'- -UGGCACGCGAGUAAGGCGUGCCAAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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