miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11396 5' -53.4 NC_003084.1 + 53409 0.66 0.931614
Target:  5'- --gGCCgCCGGAcaagcGCCaaUCUCCUCCAa -3'
miRNA:   3'- uagUGG-GGCUUu----UGGcaAGAGGAGGU- -5'
11396 5' -53.4 NC_003084.1 + 99859 0.67 0.908167
Target:  5'- uUCGCCCgGGAAAac---CUCCUCCAa -3'
miRNA:   3'- uAGUGGGgCUUUUggcaaGAGGAGGU- -5'
11396 5' -53.4 NC_003084.1 + 84111 0.67 0.901656
Target:  5'- uGUCACCCUuu-AGCuucuCGaUCUCCUCCAa -3'
miRNA:   3'- -UAGUGGGGcuuUUG----GCaAGAGGAGGU- -5'
11396 5' -53.4 NC_003084.1 + 47278 0.7 0.746788
Target:  5'- uUUugCCCGGucGgCGUaCUCCUCCAg -3'
miRNA:   3'- uAGugGGGCUuuUgGCAaGAGGAGGU- -5'
11396 5' -53.4 NC_003084.1 + 85960 1.07 0.004434
Target:  5'- cAUCACCCCGAAAACCGUUCUCCUCCAg -3'
miRNA:   3'- -UAGUGGGGCUUUUGGCAAGAGGAGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.