Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11397 | 5' | -53 | NC_003084.1 | + | 66948 | 0.66 | 0.949535 |
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Target: 5'- aGCUCGGGUguacACGcGCCAGCaGCa-- -3' miRNA: 3'- gCGGGUCCAaa--UGCaCGGUUG-CGaac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 34675 | 0.66 | 0.945015 |
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Target: 5'- gCGCUCAGGU---CGUgGCCGGgGCUg- -3' miRNA: 3'- -GCGGGUCCAaauGCA-CGGUUgCGAac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 62550 | 0.66 | 0.935199 |
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Target: 5'- gGUCaCGGaGUacgUUGCGUGaCCAACGCUg- -3' miRNA: 3'- gCGG-GUC-CA---AAUGCAC-GGUUGCGAac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 91094 | 0.67 | 0.912428 |
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Target: 5'- gGCgCCAGGgccacacgUugGUGUCcgUGCUUGg -3' miRNA: 3'- gCG-GGUCCaa------AugCACGGuuGCGAAC- -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 89586 | 0.68 | 0.862735 |
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Target: 5'- uCGCCCgAGGUUUugGgugGCCGgaggcaACGUUa- -3' miRNA: 3'- -GCGGG-UCCAAAugCa--CGGU------UGCGAac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 76222 | 0.7 | 0.782952 |
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Target: 5'- uGCCCAGGUgagggaaaAUGUGCCGAgauuggugguCGCUg- -3' miRNA: 3'- gCGGGUCCAaa------UGCACGGUU----------GCGAac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 8237 | 0.75 | 0.511291 |
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Target: 5'- gGUCCGGGUagUACGggcaaaUGCCAACGCUg- -3' miRNA: 3'- gCGGGUCCAa-AUGC------ACGGUUGCGAac -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 20685 | 0.77 | 0.378794 |
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Target: 5'- gGCgCUGGaGUUUGCGUGCCGuuGCGCUUGc -3' miRNA: 3'- gCG-GGUC-CAAAUGCACGGU--UGCGAAC- -5' |
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| 11397 | 5' | -53 | NC_003084.1 | + | 88616 | 1.08 | 0.004353 |
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Target: 5'- uCGCCCAGGUUUACGUGCCAACGCUUGc -3' miRNA: 3'- -GCGGGUCCAAAUGCACGGUUGCGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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