Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11398 | 3' | -56.6 | NC_003084.1 | + | 48063 | 0.66 | 0.816736 |
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Target: 5'- gGGGUGuG-CGUCGAG--GAGUGGUGCUa -3' miRNA: 3'- -UCCAC-CgGCAGUUCaaCUCGCCGUGA- -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 92487 | 0.66 | 0.816736 |
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Target: 5'- cGGcgGcGCUGUUggGUUGAaGUGGUACa -3' miRNA: 3'- uCCa-C-CGGCAGuuCAACU-CGCCGUGa -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 81988 | 0.66 | 0.80776 |
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Target: 5'- uGGU-GCa--CGAGUUGAGCGGUACc -3' miRNA: 3'- uCCAcCGgcaGUUCAACUCGCCGUGa -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 49198 | 0.66 | 0.798615 |
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Target: 5'- uGG-GGCCGUC--GUUG-GUGGUGCUa -3' miRNA: 3'- uCCaCCGGCAGuuCAACuCGCCGUGA- -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 96642 | 0.66 | 0.798615 |
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Target: 5'- gAGGUGGUgGUUggGc-GAGCguucguGGCGCUa -3' miRNA: 3'- -UCCACCGgCAGuuCaaCUCG------CCGUGA- -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 68640 | 0.66 | 0.798615 |
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Target: 5'- uGG-GGCCGUC--GUUG-GUGGUGCUa -3' miRNA: 3'- uCCaCCGGCAGuuCAACuCGCCGUGA- -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 79693 | 0.67 | 0.740748 |
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Target: 5'- aAGGUGGCUGU--GGU--AGCGGCGgUg -3' miRNA: 3'- -UCCACCGGCAguUCAacUCGCCGUgA- -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 54572 | 0.68 | 0.668834 |
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Target: 5'- cGGGUGGuuGUCGagcagcAGUUGua-GGCGCa -3' miRNA: 3'- -UCCACCggCAGU------UCAACucgCCGUGa -5' |
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| 11398 | 3' | -56.6 | NC_003084.1 | + | 93703 | 1.08 | 0.001727 |
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Target: 5'- gAGGUGGCCGUCAAGUUGAGCGGCACUa -3' miRNA: 3'- -UCCACCGGCAGUUCAACUCGCCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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