Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11401 | 3' | -53.8 | NC_003084.1 | + | 96263 | 0.67 | 0.901836 |
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Target: 5'- uGACGAGGgaCCAAaugUGUGGAGUucguagcagcaccacACCGc -3' miRNA: 3'- -CUGCUCC--GGUUguaACGCCUCA---------------UGGC- -5' |
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| 11401 | 3' | -53.8 | NC_003084.1 | + | 60897 | 0.67 | 0.88388 |
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Target: 5'- uACGAGGCCAACAgguuccucGAGUucACCGa -3' miRNA: 3'- cUGCUCCGGUUGUaacgc---CUCA--UGGC- -5' |
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| 11401 | 3' | -53.8 | NC_003084.1 | + | 379 | 0.68 | 0.83812 |
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Target: 5'- cACGAGGCgGugGUcgaUGUGGuuaaaAGUACCGu -3' miRNA: 3'- cUGCUCCGgUugUA---ACGCC-----UCAUGGC- -5' |
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| 11401 | 3' | -53.8 | NC_003084.1 | + | 86311 | 0.68 | 0.829503 |
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Target: 5'- --aGAGGCCAagaacaACAggcUGUGGAGUACg- -3' miRNA: 3'- cugCUCCGGU------UGUa--ACGCCUCAUGgc -5' |
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| 11401 | 3' | -53.8 | NC_003084.1 | + | 64687 | 0.69 | 0.774053 |
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Target: 5'- cACGGGGCCAcuugcugcaACGUUuCGGGGUcuauACCGa -3' miRNA: 3'- cUGCUCCGGU---------UGUAAcGCCUCA----UGGC- -5' |
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| 11401 | 3' | -53.8 | NC_003084.1 | + | 102623 | 1.08 | 0.00375 |
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Target: 5'- gGACGAGGCCAACAUUGCGGAGUACCGc -3' miRNA: 3'- -CUGCUCCGGUUGUAACGCCUCAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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