Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11402 | 5' | -45 | NC_003084.1 | + | 76946 | 0.66 | 0.99997 |
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Target: 5'- aGCGgGUgcAACGUGUGGUccucggcCGAGGUg- -3' miRNA: 3'- -CGCgCAauUUGCGUAUCA-------GCUUCAaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 15887 | 0.66 | 0.99996 |
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Target: 5'- cCGCGUUcagguGGCGCAacaUGGUCGucGUg- -3' miRNA: 3'- cGCGCAAu----UUGCGU---AUCAGCuuCAaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 32070 | 0.67 | 0.999714 |
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Target: 5'- cUGCGUUGuAGCGCcgGGUCGGGc--- -3' miRNA: 3'- cGCGCAAU-UUGCGuaUCAGCUUcaaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 25597 | 0.68 | 0.999511 |
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Target: 5'- aGCGCGgucucgcuauacUGAugGCGUGGUCGGc---- -3' miRNA: 3'- -CGCGCa-----------AUUugCGUAUCAGCUucaaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 87554 | 0.69 | 0.998954 |
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Target: 5'- aGCGCGUUAAGCGCcaGUuuacccaaccccgaaAGgCGggGUa- -3' miRNA: 3'- -CGCGCAAUUUGCG--UA---------------UCaGCuuCAaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 30327 | 0.69 | 0.997924 |
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Target: 5'- uGCGUGUUGauGACGguUGGgCGAAGg-- -3' miRNA: 3'- -CGCGCAAU--UUGCguAUCaGCUUCaaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 77296 | 0.7 | 0.99641 |
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Target: 5'- gGC-CGUUGAAUGCG-GGUCGGAGg-- -3' miRNA: 3'- -CGcGCAAUUUGCGUaUCAGCUUCaaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 107174 | 0.72 | 0.981782 |
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Target: 5'- uGCGCuGUacGAGCGCAcGGUCGAGGa-- -3' miRNA: 3'- -CGCG-CAa-UUUGCGUaUCAGCUUCaaa -5' |
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| 11402 | 5' | -45 | NC_003084.1 | + | 102927 | 1.1 | 0.022562 |
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Target: 5'- gGCGCGUUAAACGCAUAGUCGAAGUUUa -3' miRNA: 3'- -CGCGCAAUUUGCGUAUCAGCUUCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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