Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11406 | 3' | -59.6 | NC_003085.1 | + | 12415 | 0.66 | 0.435848 |
Target: 5'- -cCGGCGGGGCCGCuGCGcuccgaggaGGCuGUgUCGu -3' miRNA: 3'- aaGUCGCCUCGGUG-CGC---------CCGuCA-AGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 20600 | 0.66 | 0.435848 |
Target: 5'- cUCGGCaGcGCCGCGgaaguCGGGCGGcgCGg -3' miRNA: 3'- aAGUCGcCuCGGUGC-----GCCCGUCaaGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 48734 | 0.66 | 0.434892 |
Target: 5'- -cCAGCGGAGgCgacugagGCGCGGGUGGa--- -3' miRNA: 3'- aaGUCGCCUCgG-------UGCGCCCGUCaagc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 46714 | 0.66 | 0.398554 |
Target: 5'- -cCAGCGGAGUC-CuuGGGCAGcgccUUCGc -3' miRNA: 3'- aaGUCGCCUCGGuGcgCCCGUC----AAGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 27622 | 0.67 | 0.38069 |
Target: 5'- gUguGUGGAGCCugGgGGGC---UCGc -3' miRNA: 3'- aAguCGCCUCGGugCgCCCGucaAGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 32126 | 0.67 | 0.371961 |
Target: 5'- cUCGGUGGuGCgACGgGGGCGGc--- -3' miRNA: 3'- aAGUCGCCuCGgUGCgCCCGUCaagc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 40674 | 0.67 | 0.371961 |
Target: 5'- cUC-GCGGAGCCGC-CGGcGCAGg--- -3' miRNA: 3'- aAGuCGCCUCGGUGcGCC-CGUCaagc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 18095 | 0.68 | 0.3296 |
Target: 5'- -gCGGcCGGGGUCgagcuucuugucgGCGCGGGCGGgaCGg -3' miRNA: 3'- aaGUC-GCCUCGG-------------UGCGCCCGUCaaGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 46936 | 0.68 | 0.321723 |
Target: 5'- gUUCGGCgcgcgcaGGGGCCgACGCGGGCAc---- -3' miRNA: 3'- -AAGUCG-------CCUCGG-UGCGCCCGUcaagc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 34295 | 0.68 | 0.314755 |
Target: 5'- gUUCcGCcGAGCguCGCGGGCAGggCa -3' miRNA: 3'- -AAGuCGcCUCGguGCGCCCGUCaaGc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 11778 | 0.71 | 0.209597 |
Target: 5'- -aCGGCGGcGCCGCGCuccGGGCGugUCGg -3' miRNA: 3'- aaGUCGCCuCGGUGCG---CCCGUcaAGC- -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 4858 | 0.71 | 0.188531 |
Target: 5'- -gCGGCGGGGCCuuuCGUGGGaCGGggCa -3' miRNA: 3'- aaGUCGCCUCGGu--GCGCCC-GUCaaGc -5' |
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11406 | 3' | -59.6 | NC_003085.1 | + | 48406 | 1.07 | 0.000401 |
Target: 5'- aUUCAGCGGAGCCACGCGGGCAGUUCGg -3' miRNA: 3'- -AAGUCGCCUCGGUGCGCCCGUCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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