Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11407 | 3' | -54.8 | NC_003085.1 | + | 38208 | 0.66 | 0.710625 |
Target: 5'- -cUCGGGCgGCgguggCUCGUGCACuCUCGa- -3' miRNA: 3'- aaGGCCUG-CGa----GAGUAUGUG-GAGCag -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 30671 | 0.66 | 0.699635 |
Target: 5'- ---aGGGCGCUCU---GCGCCUCG-Ca -3' miRNA: 3'- aaggCCUGCGAGAguaUGUGGAGCaG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 27711 | 0.66 | 0.688576 |
Target: 5'- gUCCGccCGCUgUC--GCGCCUCGUUg -3' miRNA: 3'- aAGGCcuGCGAgAGuaUGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 27456 | 0.66 | 0.6663 |
Target: 5'- --aUGGGCGCgcagUUUCGUGCgggaggccACCUCGUCg -3' miRNA: 3'- aagGCCUGCG----AGAGUAUG--------UGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 9731 | 0.66 | 0.6663 |
Target: 5'- cUUCCGG-CGCUggucCUCAUcCACCUUG-Cg -3' miRNA: 3'- -AAGGCCuGCGA----GAGUAuGUGGAGCaG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 28194 | 0.67 | 0.621438 |
Target: 5'- gUCUGGGCGCgg-CAc-CACCUUGUCc -3' miRNA: 3'- aAGGCCUGCGagaGUauGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 17544 | 0.68 | 0.543655 |
Target: 5'- -aCCGGGCuGCUgcucccgugcCUCGUcCGCCUCGUUc -3' miRNA: 3'- aaGGCCUG-CGA----------GAGUAuGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 616 | 0.69 | 0.521954 |
Target: 5'- gUCCuGGGCGCgCUCGagGCuggcgcggACCUCGUCg -3' miRNA: 3'- aAGG-CCUGCGaGAGUa-UG--------UGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 24121 | 0.69 | 0.500597 |
Target: 5'- gUCCGGaaGCGCgcgC-CAUcCACCUUGUCg -3' miRNA: 3'- aAGGCC--UGCGa--GaGUAuGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 4658 | 0.71 | 0.409956 |
Target: 5'- -cCCGGugGCagaggcgagccUUUCAcggcgccgaguUGCGCCUCGUCg -3' miRNA: 3'- aaGGCCugCG-----------AGAGU-----------AUGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 39493 | 0.72 | 0.346929 |
Target: 5'- -gUCGaGGCGCUCgagaagccgGUGCGCCUCGUCg -3' miRNA: 3'- aaGGC-CUGCGAGag-------UAUGUGGAGCAG- -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 48812 | 0.74 | 0.262549 |
Target: 5'- gUCCGGGCGgUgCUCGaGCGCCUCGa- -3' miRNA: 3'- aAGGCCUGCgA-GAGUaUGUGGAGCag -5' |
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11407 | 3' | -54.8 | NC_003085.1 | + | 48066 | 1.06 | 0.001414 |
Target: 5'- cUUCCGGACGCUCUCAUACACCUCGUCc -3' miRNA: 3'- -AAGGCCUGCGAGAGUAUGUGGAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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