Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11412 | 5' | -57.7 | NC_003085.1 | + | 24388 | 0.66 | 0.532447 |
Target: 5'- cAAUgGCCUGgacGGGGCcgUGCCGgUGGAc -3' miRNA: 3'- -UUAgCGGGC---UCCCGuaGUGGCgAUCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 892 | 0.66 | 0.511249 |
Target: 5'- --gCGUCCGAgGGGCAUC-CCGUg--- -3' miRNA: 3'- uuaGCGGGCU-CCCGUAGuGGCGaucu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 12409 | 0.67 | 0.48014 |
Target: 5'- -cUCGaCCCGgcGGGGCcgCugCGCUccgaGGAg -3' miRNA: 3'- uuAGC-GGGC--UCCCGuaGugGCGA----UCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 20341 | 0.67 | 0.480139 |
Target: 5'- cAAUCGCCUGGuuccgcgccGCGUCGCCGCUGc- -3' miRNA: 3'- -UUAGCGGGCUcc-------CGUAGUGGCGAUcu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 14113 | 0.67 | 0.469975 |
Target: 5'- aAAUCGCCCacugccacGGCAUCACCGUg--- -3' miRNA: 3'- -UUAGCGGGcuc-----CCGUAGUGGCGaucu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 23539 | 0.67 | 0.468964 |
Target: 5'- -uUCGCCuucuccaCGAGGGCAcgCACCGgcccaaucuuCUGGAg -3' miRNA: 3'- uuAGCGG-------GCUCCCGUa-GUGGC----------GAUCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 42498 | 0.67 | 0.449983 |
Target: 5'- ---aGCCaacggaGAGGGCAUCGCUGCc--- -3' miRNA: 3'- uuagCGGg-----CUCCCGUAGUGGCGaucu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 7458 | 0.68 | 0.375046 |
Target: 5'- --aUGcCCCGGuGGGCGUCGCgccucaCGCUGGAg -3' miRNA: 3'- uuaGC-GGGCU-CCCGUAGUG------GCGAUCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 4960 | 0.68 | 0.375045 |
Target: 5'- ---aGCCCGAGGGCGUCuuuUCGUa--- -3' miRNA: 3'- uuagCGGGCUCCCGUAGu--GGCGaucu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 46891 | 0.69 | 0.366289 |
Target: 5'- --gUGCCCGuGGGCuugugaaggucGUCACCGCggcAGGc -3' miRNA: 3'- uuaGCGGGCuCCCG-----------UAGUGGCGa--UCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 25004 | 0.69 | 0.349203 |
Target: 5'- --aCGUCCGcGGuGCAgaaggCGCCGCUGGGc -3' miRNA: 3'- uuaGCGGGCuCC-CGUa----GUGGCGAUCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 32842 | 0.69 | 0.340876 |
Target: 5'- ---gGCCCGAGaGGCcgcgCGCCGCgGGAc -3' miRNA: 3'- uuagCGGGCUC-CCGua--GUGGCGaUCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 48988 | 0.7 | 0.301414 |
Target: 5'- -cUCGCCCGuGGGCAccugcaacagCGCCgGCUGGc -3' miRNA: 3'- uuAGCGGGCuCCCGUa---------GUGG-CGAUCu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 27099 | 0.7 | 0.286646 |
Target: 5'- --aCGUCCGAGGGCAUCugggacguuuCCGCg--- -3' miRNA: 3'- uuaGCGGGCUCCCGUAGu---------GGCGaucu -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 4940 | 0.71 | 0.279478 |
Target: 5'- gAGUCGUCCGGcgggugcuGGGCAcCACCGCgcAGAa -3' miRNA: 3'- -UUAGCGGGCU--------CCCGUaGUGGCGa-UCU- -5' |
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11412 | 5' | -57.7 | NC_003085.1 | + | 46654 | 1.07 | 0.000633 |
Target: 5'- gAAUCGCCCGAGGGCAUCACCGCUAGAg -3' miRNA: 3'- -UUAGCGGGCUCCCGUAGUGGCGAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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