Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11413 | 3' | -59.1 | NC_003085.1 | + | 17014 | 0.66 | 0.512214 |
Target: 5'- uCCaugaGGCCGC---GGGCGUucGGUCCAAc -3' miRNA: 3'- -GGg---CCGGCGgaaCUCGCA--CCAGGUUc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 47952 | 0.66 | 0.502019 |
Target: 5'- gCCUGGCgGCCgUGAGgCGcaccGG-CCGAGg -3' miRNA: 3'- -GGGCCGgCGGaACUC-GCa---CCaGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 46632 | 0.66 | 0.491914 |
Target: 5'- gCCCGGCUGCCggGcAGaCGUGuugCCAGu -3' miRNA: 3'- -GGGCCGGCGGaaC-UC-GCACca-GGUUc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 46250 | 0.66 | 0.491914 |
Target: 5'- gCCGGCauCGCCUUGcGGCaucgGG-CCAGGg -3' miRNA: 3'- gGGCCG--GCGGAAC-UCGca--CCaGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 5717 | 0.66 | 0.481904 |
Target: 5'- aCUCGGCCGUCUacucGCG-GG-CCGAGg -3' miRNA: 3'- -GGGCCGGCGGAacu-CGCaCCaGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 697 | 0.66 | 0.471993 |
Target: 5'- gUCCGGCCucgggcgcgGCC-UGAGCa-GGUCCAc- -3' miRNA: 3'- -GGGCCGG---------CGGaACUCGcaCCAGGUuc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 13782 | 0.66 | 0.471993 |
Target: 5'- aCCuGCC-CCUUGAGCagaaucaGGUCCGGGu -3' miRNA: 3'- gGGcCGGcGGAACUCGca-----CCAGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 35146 | 0.67 | 0.42408 |
Target: 5'- gCCGGCUGCCgcaacaUGAGCGgcaggaGGUucCCGAu -3' miRNA: 3'- gGGCCGGCGGa-----ACUCGCa-----CCA--GGUUc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 36655 | 0.67 | 0.42408 |
Target: 5'- gCCCGGaCCGUCa-GGGCGUGGagCAc- -3' miRNA: 3'- -GGGCC-GGCGGaaCUCGCACCagGUuc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 29108 | 0.68 | 0.405745 |
Target: 5'- aUCCGGCCGCUcccgcgcUGAGgcgcCGUGGcCCAGa -3' miRNA: 3'- -GGGCCGGCGGa------ACUC----GCACCaGGUUc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 3980 | 0.68 | 0.395875 |
Target: 5'- -aCGGCCGCgCUUgcGAGCGcgacacggcacagUGGcCCAAGg -3' miRNA: 3'- ggGCCGGCG-GAA--CUCGC-------------ACCaGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 27651 | 0.68 | 0.37061 |
Target: 5'- gCUCGGCgGCCUUGGGCGccaacuccuuGUCgAAGg -3' miRNA: 3'- -GGGCCGgCGGAACUCGCac--------CAGgUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 43789 | 0.68 | 0.362157 |
Target: 5'- aCCGGCCaGCgUcaUGAGCGU-GUCCGu- -3' miRNA: 3'- gGGCCGG-CGgA--ACUCGCAcCAGGUuc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 30972 | 0.69 | 0.337611 |
Target: 5'- gCUGGCCGCCUUGAGgaCGggGGcUgCGAGu -3' miRNA: 3'- gGGCCGGCGGAACUC--GCa-CC-AgGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 8655 | 0.69 | 0.314298 |
Target: 5'- gCCGGCCgggacgcgcgugGCCUUGGGCGccggGGUgCCGu- -3' miRNA: 3'- gGGCCGG------------CGGAACUCGCa---CCA-GGUuc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 45114 | 0.7 | 0.299444 |
Target: 5'- uCCUGGCCGCUcaccgUGuGCugccGGUCCAGGu -3' miRNA: 3'- -GGGCCGGCGGa----ACuCGca--CCAGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 45073 | 0.7 | 0.298716 |
Target: 5'- gUCCGGuauCCGCCguccacaacuccuucGCGUGGUCCAGGu -3' miRNA: 3'- -GGGCC---GGCGGaacu-----------CGCACCAGGUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 42866 | 0.72 | 0.205093 |
Target: 5'- uCCCGGCCcgGCCggGGGCGUG-UCgAGGg -3' miRNA: 3'- -GGGCCGG--CGGaaCUCGCACcAGgUUC- -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 14920 | 0.73 | 0.175187 |
Target: 5'- aCCGGCCGCCagc-GCGUGGcgcUCCAAc -3' miRNA: 3'- gGGCCGGCGGaacuCGCACC---AGGUUc -5' |
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11413 | 3' | -59.1 | NC_003085.1 | + | 45910 | 1.1 | 0.00031 |
Target: 5'- gCCCGGCCGCCUUGAGCGUGGUCCAAGg -3' miRNA: 3'- -GGGCCGGCGGAACUCGCACCAGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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