Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11414 | 5' | -51.1 | NC_003085.1 | + | 36811 | 0.66 | 0.867991 |
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Target: 5'- uCGUCCCGcACGCucuucagCGAGG-CAGCg -3' miRNA: 3'- cGUAGGGU-UGCGuuga---GCUCCaGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 10022 | 0.66 | 0.867991 |
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Target: 5'- uGCcgCCCGaguccuuuGCGCcAUgCGAGGUCGAg -3' miRNA: 3'- -CGuaGGGU--------UGCGuUGaGCUCCAGUUg -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 16443 | 0.66 | 0.867991 |
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Target: 5'- gGCG--CCGACGUcAUUCGAGG-CGACg -3' miRNA: 3'- -CGUagGGUUGCGuUGAGCUCCaGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 15568 | 0.66 | 0.867991 |
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Target: 5'- cGCGUCaCUggUGCGAUUUcagGAGGUCGc- -3' miRNA: 3'- -CGUAG-GGuuGCGUUGAG---CUCCAGUug -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 614 | 0.66 | 0.876166 |
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Target: 5'- cCGUCCUgGGCGC-GCUCGAGGcUGGCg -3' miRNA: 3'- cGUAGGG-UUGCGuUGAGCUCCaGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 8009 | 0.66 | 0.883291 |
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Target: 5'- cGCGcCCCAgccuugcGCGcCAACUCGAcGGagAGCg -3' miRNA: 3'- -CGUaGGGU-------UGC-GUUGAGCU-CCagUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 10848 | 0.66 | 0.891693 |
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Target: 5'- aGCGUCCCGACG-AGCaCGGcGGUgcaugCAACa -3' miRNA: 3'- -CGUAGGGUUGCgUUGaGCU-CCA-----GUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 26697 | 0.66 | 0.891693 |
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Target: 5'- cCGUCgCCAcCGCGGCgggCGucGUCGGCg -3' miRNA: 3'- cGUAG-GGUuGCGUUGa--GCucCAGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 7269 | 0.66 | 0.899033 |
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Target: 5'- uGUcgCCCAGCGCcucauGC-CGAGGcuggcugCGACg -3' miRNA: 3'- -CGuaGGGUUGCGu----UGaGCUCCa------GUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 32406 | 0.66 | 0.899033 |
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Target: 5'- cGUAcuUCUCGACGCccucgaAGCUCcgGAGGUUGGCg -3' miRNA: 3'- -CGU--AGGGUUGCG------UUGAG--CUCCAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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