Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11416 | 3' | -43.3 | NC_003085.1 | + | 7913 | 0.66 | 0.999682 |
Target: 5'- gGCGAgCGCGuCCUgucgGcgUGCAccaacucGUUGCg -3' miRNA: 3'- -CGUUaGCGUuGGAa---CuaACGU-------UAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 27003 | 0.66 | 0.999597 |
Target: 5'- cGCGAguggUGCAcggGCCUUGGUgUGCc--UGCg -3' miRNA: 3'- -CGUUa---GCGU---UGGAACUA-ACGuuaACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 25166 | 0.66 | 0.999597 |
Target: 5'- gGCGAcCGUGGCCU-GGUgGCGGUgGCg -3' miRNA: 3'- -CGUUaGCGUUGGAaCUAaCGUUAaCG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 33694 | 0.67 | 0.999137 |
Target: 5'- cGCGuugaCGCGcucaaacGCCUcGAUUGCAGcgGCa -3' miRNA: 3'- -CGUua--GCGU-------UGGAaCUAACGUUaaCG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 33 | 0.67 | 0.998672 |
Target: 5'- aCAGUaCGCAcauGCCUUGcuaucUGCAAcUUGCa -3' miRNA: 3'- cGUUA-GCGU---UGGAACua---ACGUU-AACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 9123 | 0.68 | 0.99797 |
Target: 5'- uGCuGUCGCGACCUcucUGGgagcgGCAccugGCg -3' miRNA: 3'- -CGuUAGCGUUGGA---ACUaa---CGUuaa-CG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 1549 | 0.68 | 0.997515 |
Target: 5'- uGCuGUCGCGagGCCgUGcgcGUcgccUGCAGUUGCg -3' miRNA: 3'- -CGuUAGCGU--UGGaAC---UA----ACGUUAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 606 | 0.68 | 0.996979 |
Target: 5'- uGCAagcccGUCGCGGCCUacggcgGGUUGUgc-UGCg -3' miRNA: 3'- -CGU-----UAGCGUUGGAa-----CUAACGuuaACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 12221 | 0.69 | 0.995618 |
Target: 5'- uGCGA-CGCcuGCCUUGAgcGCGGcgGCu -3' miRNA: 3'- -CGUUaGCGu-UGGAACUaaCGUUaaCG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 40554 | 0.69 | 0.991404 |
Target: 5'- gGCGucgaggCGCAagGCCUUGGUgGCGG-UGCg -3' miRNA: 3'- -CGUua----GCGU--UGGAACUAaCGUUaACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 38229 | 0.7 | 0.989963 |
Target: 5'- cGCGGUagUGCAccACCUUGGgcUGaCGGUUGCa -3' miRNA: 3'- -CGUUA--GCGU--UGGAACUa-AC-GUUAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 5149 | 0.7 | 0.984489 |
Target: 5'- gGCAuccauGUCGCAacACCUcGcg-GCGAUUGCu -3' miRNA: 3'- -CGU-----UAGCGU--UGGAaCuaaCGUUAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 10926 | 0.73 | 0.944646 |
Target: 5'- aGCAAggGCuuGCCggGGUUGguGUUGCg -3' miRNA: 3'- -CGUUagCGu-UGGaaCUAACguUAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 15334 | 0.75 | 0.899249 |
Target: 5'- cGCGAUUGCAACCauacUGGUaacUGCA--UGCg -3' miRNA: 3'- -CGUUAGCGUUGGa---ACUA---ACGUuaACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 4579 | 0.92 | 0.163984 |
Target: 5'- uGCAAUUGCAAUCaaGGUUGCGAUUGCg -3' miRNA: 3'- -CGUUAGCGUUGGaaCUAACGUUAACG- -5' |
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11416 | 3' | -43.3 | NC_003085.1 | + | 44881 | 1.19 | 0.003962 |
Target: 5'- cGCAAUCGCAACCUUGAUUGCAAUUGCa -3' miRNA: 3'- -CGUUAGCGUUGGAACUAACGUUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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