Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11418 | 5' | -56.5 | NC_003085.1 | + | 1306 | 0.66 | 0.650267 |
Target: 5'- cUCCCGcGUGGCCGcGAAgg-CUggcaCUGCGa -3' miRNA: 3'- -AGGGCuUACCGGC-CUUagaGA----GGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 7563 | 0.69 | 0.440066 |
Target: 5'- gCCCGAgaAUGcGCUGGAGUCg--CUGCGu -3' miRNA: 3'- aGGGCU--UAC-CGGCCUUAGagaGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 8776 | 0.66 | 0.617491 |
Target: 5'- aCCCGGAcgucgucccccUGcGCCGGcgg--CUCCGCGa -3' miRNA: 3'- aGGGCUU-----------AC-CGGCCuuagaGAGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 9586 | 0.67 | 0.552509 |
Target: 5'- -gCCGAGca-CCGGcGUCUCUCCGCc -3' miRNA: 3'- agGGCUUaccGGCCuUAGAGAGGCGc -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 15040 | 0.68 | 0.508243 |
Target: 5'- cUCCGAGuuuuggcUGGCCGGAAUCcCcaugccgUCCGCc -3' miRNA: 3'- aGGGCUU-------ACCGGCCUUAGaG-------AGGCGc -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 31726 | 0.69 | 0.460586 |
Target: 5'- cUCCCGAA-GGCgGGAcuggucacgcgccucUCUCUCUGCc -3' miRNA: 3'- -AGGGCUUaCCGgCCUu--------------AGAGAGGCGc -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 34499 | 0.66 | 0.650267 |
Target: 5'- cUCCCGGAggugGGCCGccagCgCUUCGCGc -3' miRNA: 3'- -AGGGCUUa---CCGGCcuuaGaGAGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 35220 | 0.66 | 0.617491 |
Target: 5'- gCCCGGAcucuccgaUGGCCGGuugAGUCUggCUGCa -3' miRNA: 3'- aGGGCUU--------ACCGGCC---UUAGAgaGGCGc -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 38721 | 0.67 | 0.552509 |
Target: 5'- gCCCGuGUGGCCGGAcagCaacagCgUCCGCu -3' miRNA: 3'- aGGGCuUACCGGCCUua-Ga----G-AGGCGc -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 40027 | 0.76 | 0.180464 |
Target: 5'- uUCCCGuAcgGGCCGGAcuacGUCgacCUCUGCGu -3' miRNA: 3'- -AGGGC-UuaCCGGCCU----UAGa--GAGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 41674 | 0.66 | 0.606577 |
Target: 5'- cCCUGuugGGCCGcGAAUgcgCUCgCCGCGa -3' miRNA: 3'- aGGGCuuaCCGGC-CUUA---GAGaGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 43790 | 1.1 | 0.000606 |
Target: 5'- aUCCCGAAUGGCCGGAAUCUCUCCGCGg -3' miRNA: 3'- -AGGGCUUACCGGCCUUAGAGAGGCGC- -5' |
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11418 | 5' | -56.5 | NC_003085.1 | + | 46757 | 0.72 | 0.301202 |
Target: 5'- gCCCGAAguccucGCCGGAGUCcaccaccuccgcCUCCGCGa -3' miRNA: 3'- aGGGCUUac----CGGCCUUAGa-----------GAGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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