Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11420 | 3' | -58.5 | NC_003085.1 | + | 30052 | 0.66 | 0.440807 |
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Target: 5'- cAGGUGGCGCCGcccuGgGCCUUGc- -3' miRNA: 3'- aUCCACUGCGGCcucuCgCGGAACuu -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 22595 | 0.66 | 0.431068 |
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Target: 5'- ----cGGCGCCGGcAGGuGCGCCUccUGAAu -3' miRNA: 3'- auccaCUGCGGCC-UCU-CGCGGA--ACUU- -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 13795 | 0.67 | 0.384342 |
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Target: 5'- gAGcGUGACGCCagucugcguGGAGguGGCGCCaUGAc -3' miRNA: 3'- aUC-CACUGCGG---------CCUC--UCGCGGaACUu -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 2410 | 0.67 | 0.381647 |
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Target: 5'- gGGGUucGGCGCCugaccucacccuggGGugguagauGGAGCGCCUUGAc -3' miRNA: 3'- aUCCA--CUGCGG--------------CC--------UCUCGCGGAACUu -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 18689 | 0.68 | 0.357964 |
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Target: 5'- aGGGUGACGCCGGucAGUggcccgccccGCCUgacGAGa -3' miRNA: 3'- aUCCACUGCGGCCucUCG----------CGGAa--CUU- -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 46107 | 0.68 | 0.357964 |
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Target: 5'- uUGGGcucacgGGCGUCGGGGAGCGUCg---- -3' miRNA: 3'- -AUCCa-----CUGCGGCCUCUCGCGGaacuu -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 14375 | 0.71 | 0.227026 |
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Target: 5'- gGGGUGACGUCgcagauggcccgGGAGAGgcgggagcgcauCGCCUUGGAg -3' miRNA: 3'- aUCCACUGCGG------------CCUCUC------------GCGGAACUU- -5' |
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| 11420 | 3' | -58.5 | NC_003085.1 | + | 41169 | 1.04 | 0.000764 |
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Target: 5'- gUAGGUGACGCCGGAGAGCGCCUUGAAc -3' miRNA: 3'- -AUCCACUGCGGCCUCUCGCGGAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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