Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11421 | 5' | -57.1 | NC_003085.1 | + | 36029 | 0.66 | 0.582905 |
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Target: 5'- cGGCcaugGGCacgCCGuaUGCCCccgUCUUCCg -3' miRNA: 3'- -CCGa---CCGa--GGCcgGUGGGaa-AGAAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 36727 | 0.66 | 0.572083 |
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Target: 5'- cGCaccacCUCCGGCC-CCUUUUCUUgCCg -3' miRNA: 3'- cCGacc--GAGGCCGGuGGGAAAGAA-GG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 37889 | 0.67 | 0.53994 |
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Target: 5'- uGCUGGUgggcaccacgCCGacggcGCUGCCCU-UCUUCCg -3' miRNA: 3'- cCGACCGa---------GGC-----CGGUGGGAaAGAAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 39205 | 0.67 | 0.498096 |
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Target: 5'- cGCaGGCUCCGGUacggACCCccUCggCCa -3' miRNA: 3'- cCGaCCGAGGCCGg---UGGGaaAGaaGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 40126 | 0.68 | 0.445036 |
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Target: 5'- uGCUGGUcCUGGCCGCCgCgcgUCUgagucgcucaacccUCCa -3' miRNA: 3'- cCGACCGaGGCCGGUGG-Gaa-AGA--------------AGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 41098 | 1.15 | 0.000232 |
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Target: 5'- cGGCUGGCUCCGGCCACCCUUUCUUCCa -3' miRNA: 3'- -CCGACCGAGGCCGGUGGGAAAGAAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 45357 | 0.7 | 0.355135 |
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Target: 5'- uGGCUGGCagccuggaccgcCCGGcCCACCCagaaCUUCUg -3' miRNA: 3'- -CCGACCGa-----------GGCC-GGUGGGaaa-GAAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 45898 | 0.67 | 0.518851 |
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Target: 5'- aGGCgcgcuUGcGC-CCGGCCGCCUUgagCguggUCCa -3' miRNA: 3'- -CCG-----AC-CGaGGCCGGUGGGAaa-Ga---AGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 48802 | 0.69 | 0.419248 |
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Target: 5'- cGCggGGCUCaCGGCagCACCuCUUUCgcgCCg -3' miRNA: 3'- cCGa-CCGAG-GCCG--GUGG-GAAAGaa-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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